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  • 1
    ISSN: 1434-0879
    Keywords: Urease-induced crystallisation ; Coulter counter ; Undiluted human urine ; Magnesium ammonium phosphate ; Calcium phosphate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Urease-induced crystallisation was studied in different human urine samples after urease incubation. The studies were performed using the Coulter counter technique, which enables determination of the number and size of particles in a solution and calculation of the total particle volume. The crystallization took place in three consecutive but overlapping steps: (1) nucleation, (2) growth and (3) aggregation. The maximal number of particles obtained in the different samples varied little, but there was a great variation in particle size and total particle volume. The variation in particle size appeared to be mainly due to differences in particle growth, a factor that might be of importance for stone formation.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Urological research 18 (1990), S. 407-411 
    ISSN: 1434-0879
    Keywords: Urease-induced crystallization ; Serum ; Albumin ; Gammaglobulin ; Magnesium ammonium phosphate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The effects of serum, albumin and gammaglobulins on urease-induced crystallization have been studied in synthetic and in human urine. Serum and the studied proteins increased urease enzymatic activity in synthetic urine. In human urine only serum had this effect. In synthetic urine, the proteins and serum markedly decreased the precipitation attached to glass surfaces, while the intraluminal precipitation was increased. In human urine, similar but weaker effects on the precipitation were found for serum and albumin. These findings suggest that the proteins studied, in the concentrations in which they are present in human urine, have profound effects on urease-induced crystallization and may be physiological crystallization inhibitors.
    Type of Medium: Electronic Resource
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