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  • 1
    ISSN: 1432-072X
    Keywords: Thiobacillus ; Leaching ; Chemolithotrophic ; Acidophilic ; Marine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract From the shallow geothermally heated seafloor at the beach of Porto di Levante (Vulcano, Italy) 8 strains of long, tiny rods were isolated, which represent the first marine metal-mobilizing bacteria. Cells are Gram negative. They grow in a temperature range between 23 and 41°C with an optimum around 37°C at a salt concentration of up to 6.0% NaCl. The isolates are obligately chemolithotrophic, acidophilic aerobes which use sulfidic ores, elemental sulfur or ferrous iron as energy sources and procedure sulfuric acid. They show an upper pH-limit of growth at around 4.5. The G+C content of their DNA is around 64 mol%. Based on the results of the DNA-DNA hybridization they represent a new group within the genus Thiobacillus. Isolate LM3 is described as the type strain of the new species Thiobacillus prosperus.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-072X
    Keywords: Methanogens ; Archaebacteria ; Cell division ; Glycoprotein ; Acetate ; Taxonomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract An angular plate-shaped weakly motile mesophilic methanogen was isolated from a swamp of drilling waste in Italy. Growth occurs on H2/CO2 or on formate. Acetate is required in addition. The optimal doubling time is 7 h at 40° C. The cell envelope is composed most likely of glycoprotein subunits in hexagonal arrangement. The GC-content of its DNA is 47.5 mol%. On the basis of DNA-RNA hybridization it was found to represent a new family, the Methanoplanaceae within the order Methanomicrobiales.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 145 (1986), S. 56-61 
    ISSN: 1432-072X
    Keywords: Archaebacteria ; Extremely thermophilic ; Marine ; Pyrococcus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Ten strains representing a novel genus of marine thermophilic archaebacteria growing at between 70 and 103°C with an optimal growth temperature of 100°C and a doubling time of only 37 min were isolated from geothermally heated marine sediments at the beach of Porto di Levante, Vulcano, Italy. The organisms are spherical-shaped, 0.8 to 2.5 μm in width and exhibit monopolar polytrichous flagellation. They are strictly anaerobic heterotrophs, growing on starch, maltose, peptone and complex organic substrates. Only CO2 and H2 could be detected as metabolic products, the latter being inhibitory to growth at high concentrations. Hydrogen inhibition can be prevented by the addition of So, whereupon H2S is formed in addition, most likely as the result of a “detoxification” reaction. The GC-content of the DNA of isolate Vc 1 is 38 mol%. The new genus is named Pyrococcus, the “fireball”. Type species and strain is Pyrococcus furiosus Vc 1 (DSM 3638).
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1432-072X
    Keywords: Methanogens ; Archaebacteria ; Autotrophic ; Thermophilic
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract An autotrophic thermophilic motile coccoid methanogen was isolated from geothermally heated sea sediments close to Naples, Italy. Growth occurs on H2/CO2 and on formate between 30 and 70°C with an optimum at 65°C. The optimal doubling time is only 55 min. The NaCl-concentration ranges from 1.3% to 8.3% with an optimum around 4%. By its G+C-content of 31.3 mol%, its subunit envelope, and by DNA-RNA hybridization the new isolate is clearly defined to be a member of the genusMethanococcus. We name itMethanococcus thermolithotrophicus.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1432-072X
    Keywords: Methanopyrus ; Methanogens ; Archaea ; Hyperthermophilic ; Marine ; Vents
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A novel group of hyperthermophilic rod-shaped motile methanogens was isolated from a hydrothermally heated deep sea sediment (Guaymas Basin, Gulf of California) and from a shallow marine hydrothermal system (Kolbeinsey ridge, Iceland). The grew between 84 and 110°C (opt: 98°C) and from 0.2% to 4% NaCl (opt. 2%) and pH 5.5 to 7 (opt: 6.5). The isolates were obligate chemolithoautotrophes using H2/CO2 as energy and carbon sources. In the presence of sulfur, H2S was formed and cells tended to lyse. The cell wall consisted of a new type of pseudomurein containing ornithin in addition to lysine and no N-acetylglucosamine. The pseudomurein layer was covered by a detergent-sensitive protein surface layer. The core lipid consisted exclusively of phytanyl diether. The GC content of the DNA was 60 mol%. By 16S rRNA comparisons the new organisms were not related to any of the three methanogenic lineages. Based on the physiological and molecular properties of the new isolates, we describe here a new genus, which we name Methanopyrus (the “methane fire”). The type species is Methanopyrus kandleri (type strain: AV19; DSM 6324).
    Type of Medium: Electronic Resource
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