ISSN:
1432-2072
Keywords:
Chlorpromazine
;
Thioridazine
;
Metabolites
;
High performance liquid chromatography
;
Electrochemical detection
;
Human brain
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract Thirteen phenothiazine compounds were separated chromatographically using high performance liquid chromatography with coulometric electrochemical detection. These could be extracted from brain tissue using direct homogenization in tetrahydrofuran followed by one centrifugation, evaporation of supernatant and reconstitution in water. Fluphenazine was used as the internal standard. The absolute lower limit of detection was approximately 50 pg/mg wet tissue, and recovery rates for most standards added to brain homogenates were greater than 85%. Chromatograms from patients receiving chlorpromazine (600 mg) and thioridazine (600 mg) are shown and endogenous brain levels quantified. The results are discussed with respect to their relevance in schizophrenic research.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00179183
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