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  • 1
    Electronic Resource
    Electronic Resource
    The Role of Posttranscriptional Modification in Stabilization of Transfer RNA from Hyperthermophiles (1994)
    s.l. : American Chemical Society
    Biochemistry 33 (1994), S. 7869-7876 
    Details
    ISSN: 1520-4995
    Source: ACS Legacy Archives
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/bi00191a014
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  • 2
    Electronic Resource
    Electronic Resource
    Morphological, Small Subunit rRNA, and Physiological Characterization of Trimyema minutum (Kahl, 1931), an Anaerobic Ciliate from Submarine Hydrothermal Vents Growing from 28°C to 52°C (2002)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 49 (2002), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: . A thermophilic strain of Trimyema minutum was isolated from the hydrothermally heated sea floor at Vulcano Island (Italy) and cultivated monoxenically on Marinobaaer sp. and Methanococcus thermolithotrophicus. It can be propagated strictly an aerobically and is sensitive to oxygen: if exposed to air at 48°C all cells die within 60 min. It grows from 0.45–7.2% (w/v) salt and at pH 6.0–8.0. The isolate is the most extreme thermophilic ciliate which ever has been cultivated, exhibiting an optimal growth temperature of 48°C (doubling time 6 h). Growth occurs between 28°C and 52°C. Trimyema minutum is redescribed using live observation and silver impregnation. Its morphology and the small subunit ribosomal RNA sequence is distinctly different from that of T. compressum, but morphology is highly similar to that of T. shoalsiaNerad et al. 1995, which is thus probably a junior synonym of T. minutum. To stabilize the bewildering species taxonomy in Trimyema. we suggest to recognize our population as a neotype of T. minutum.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.2002.tb00527.x
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  • 3
    Electronic Resource
    Electronic Resource
    A new phylum of Archaea represented by a nanosized hyperthermophilic symbiont (2002)
    [s.l.] : Nature Publishing Group
    Nature 417 (2002), S. 63-67 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] According to small subunit ribosomal RNA (ss rRNA) sequence comparisons all known Archaea belong to the phyla Crenarchaeota, Euryarchaeota, and—indicated only by environmental DNA sequences—to the ‘Korarchaeota’. Here we report the cultivation of a new ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/417063a
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  • 4
    Electronic Resource
    Electronic Resource
    II. Fine structure of S-layers (1997)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology reviews 20 (1997), S. 0 
    Details
    ISSN: 1574-6976
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: S-layers are now considered a common cell wall structure in Bacteria and Archaea as well as in some algae. Morphological and chemical studies have revealed that S-layers consist of crystalline arrays of protein or glycoprotein subunits forming oblique, square or hexagonal lattices on the cell surface. Electron microscopy and computer image enhancement techniques have been applied to obtain structural information down to the nanometer range. This chapter deals with the wide distribution of S-layers among cyanobacteria, and their morphological and chemical characterization, and the potential of high resolution electron microscopic studies applied to the cell envelope of Pyrodictium. The occurrence of S-layers in cyanobacteria was investigated by cryomethods and ultrathin sectioning. These investigations indicate that the ultrastructure of S-layers may be exploited as an auxiliary taxonomic criterion in the classification of cyanobacteria. Pyrodictium is the first organism which has shown an optimum growth temperature above 100°C. The highly irregularly shaped, flagellated cells are interconnected by extracellular tubules. The three-dimensional structure of this network was visualized with high resolution scanning electron microscopy while the fine structure of the cell wall architecture was studied in detail with various electron microscopic techniques. Both contributions demonstrate that the investigation of the fine structure of S-layers is fundamental for establishing structure-function relationships for these two-dimensional crystalline arrays.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6976.1997.tb00302.x
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  • 5
    Electronic Resource
    Electronic Resource
    Hyperthermophilic procaryotes (1996)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology reviews 18 (1996), S. 0 
    Details
    ISSN: 1574-6976
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract: Hyperthermophilic Archaea and Bacteria with optimal growth temperatures between 80°C and 110°C have been isolated from geothermal and hydrothermal environments. By 16S rRNA sequence comparisons, they exhibit a great phylogenetic diversity indicated by 25 different genera. Hyperthermophiles consist of anaerobic and aerobic chemolithoautotrophs and heterotrophs growing at neutral or acidic pH. Based on their outstanding heat resistance they are interesting objects the same for basic research as for biotechnology.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6976.1996.tb00233.x
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  • 6
    Electronic Resource
    Electronic Resource
    Anaerobic respiration with elemental sulfur and with disulfides (1998)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology reviews 22 (1998), S. 0 
    Details
    ISSN: 1574-6976
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Anaerobic respiration with elemental sulfur/polysulfide or organic disulfides is performed by several bacteria and archaea, but has only been investigated in a few organisms in detail. The electron transport chain that catalyzes polysulfide reduction in the Gram-negative bacterium Wolinella succinogenes consists of a dehydrogenase (formate dehydrogenase or hydrogenase) and polysulfide reductase. The enzymes are integrated in the cytoplasmic membrane with the catalytic subunits exposed to the periplasm. The mechanism of electron transfer from formate dehydrogenase or hydrogenase to polysulfide reductase is discussed. The catalytic subunit of polysulfide reductase belongs to the family of molybdopterin-dinucleotide-containing oxidoreductases. From the hyperthermophilic archaeon Pyrodictium abyssi isolate TAG11 an integral membrane complex has been isolated which catalyzes the reduction of sulfur with H2 as electron donor. This enzyme complex, which is composed of a hydrogenase and a sulfur reductase, contains heme groups and several iron-sulfur clusters, but does not contain molybdenum or tungsten. In methanogenic archaea, the heterodisulfide of coenzyme M and coenzyme B is the terminal electron acceptor of the respiratory chain. In methanogens belonging to the order Methanosarcinales, this respiratory chain is composed of a dehydrogenase, the membrane-soluble electron carrier methanophenazine, and heterodisulfide reductase. The catalytic subunit of heterodisulfide reductase contains only iron-sulfur clusters. An iron-sulfur cluster may directly be involved in the reduction of the disulfide substrate.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6976.1998.tb00376.x
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  • 7
    Electronic Resource
    Electronic Resource
    The fine structure of the fibers of Pyrodictium occultum (1988)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 49 (1988), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract Depending on the culture conditions, Pyrodictium occultum cells revealed two different types of fibers with significant differences in their width in the electron microscope. During growth on elemental sulfur preferentially fibres with a diameter of about 23 nm (type I) were produced. When elemental sulfur was substituted by thiosulfate fibers with a diameter of around 15 nm (type II), were the main appendages. Both types form hollow cylinders consisting of helically arranged sub-units with a wall thickness of 2–3 nm. A triple- layered unit membrane could not be found.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1988.tb02717.x
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  • 8
    Electronic Resource
    Electronic Resource
    MvnI: A restriction enzyme in the archaebacterium Methanococcus vannielii (1988)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 52 (1988), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract The methanogenic archaebacerium Methanococcus vannielii contains a type II restriction endonuclease. The enzyme was purified by a simple three-step procedure resulting in enzyme preparations free of contaminating unspecific nucleases. The restriction enzyme recognizes and cleaves the sequence 5′-CG ↓ CG-3′ (FnuDII and ThaI isoschizomer) and generates DNA fragments with blunt ends. Due to its purity and activity at moderate temperatures, MvnI might be a useful alternative to FnuDII and ThaI active at 60°C.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1988.tb02601.x
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  • 9
    Electronic Resource
    Electronic Resource
    Lipid analysis of Methanopyrus kandleri (1996)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 136 (1996), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract Non-polar and polar lipids were isolated from Methanopyrus kandleri. Non-polar lipids accounted for 50% w/w of total lipids, with a high proportion of 2,3-di-O-geranylgeranyl-sn-glycerol, 2,3-di-O-phytanyl-sn-glycerol and geranylgeraniol. The core lipids prepared by mild acid methanolysis consisted exclusively of 2,3-di-O-phytanyl-sn-glycerol. Two-dimensional TLC showed mostly glycolipids, and minor amounts of aminophospholipids, phosphoglycolipids and phospholipids. The purification yielded three diglycosyl-lipids (50% of total polar lipids), one triglycosyl-lipid (5%) and six glycosyl-lipids with five glycosyl-groups (36%), which consisted of glucose, galactose and mannose. The lipid analysis supports the unique position of Methanopyrus kandleri within the 16S rRNA-based phylogenetic tree.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1996.tb08049.x
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  • 10
    Electronic Resource
    Electronic Resource
    Glycogen in Methanolobus and Methanococcus (1985)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 28 (1985), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract Glycogen was isolated from Methanolobus tindarius and 4 species of Methanococcus. It was identified by the iodine reaction, determination of glucose, maltose and isomaltose in acid hydrolysates and partial acid hydrolysates, respectively, determination of erythritol and glycerol after periodate oxidation and treatment with amyloglucosidase. Glycogen particles in the cells are distributed throughout the cytoplasm. Ml. tindarius can use elemental nitrogen and cysteine as nitrogen source. The glycogen and polyphosphate content of cells of Ml. tindarius increased under nitrogen limitation. During incubation without external energy source, glycogen decreased and methane formation was correlated to the glycogen content of the cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1985.tb00803.x
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