ISSN:
1432-2013
Keywords:
Key words Neutrophils
;
Reperfusion injury
;
Microspheres
;
Coronary resistance
;
Microcirculation
;
Leucocyte adhesion
;
CD18
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract Effects of microspheres (5 μm or 10 μm diameter) and polymorphonuclear leucocytes (PMN) on coronary resistance were compared in beating, non-working isolated guinea-pig hearts (Langendorff preparation). The hearts were buffer perfused (5 ml/min, constant flow) and particles or cells were infused into the coronary system as a bolus (1 ml, 1 min). Coronary perfusion pressure, coronary flow and formation of epicardial transudate were measured before and after bolus administration. Coronary resistance was estimated from these parameters. Retention of particles or cells was monitored by quantifying the numbers emerging in the coronary effluent in relation to the number administered. The effects of PMN were also studied after 15 min of global ischaemia. Coronary resistance correlated with the number of 10-μm particles infused, which were almost quantitatively retained. In contrast, 5-μm beads had no such effect and were not retained in the coronary system. Though considerable numbers of PMN were retained in the hearts (about 21% under control conditions and 35% after ischaemia), coronary resistance was not increased in either case. Blockage of the CD18 adhesion complex by monoclonal antibodies lowered basal retention to 11% and completely prevented the elevation of retention by ischaemia. We conclude that, in this experimental model, PMN, permanently retained in the hearts under normal flow conditions and especially after brief ischaemia, do not cause acute, haemodynamically relevant capillary plugging, but adhere to postcapillary venules via CD18.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s004240050336
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