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  • 1
    Electronic Resource
    Electronic Resource
    The inhibitory effect of phenothiazine stains on rat and guinea-pig mast cell damage induced by antihistamines and compound 48/80 (1973)
    Springer
    Naunyn-Schmiedeberg's archives of pharmacology 279 (1973), S. 173-184 
    Details
    ISSN: 1432-1912
    Keywords: Mast Cells ; Antihistamines ; Dyes ; Stains
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary 1. Toluidine blue inhibits rat mesentery mast cell disruption induced by compound 48/80 and by the antihistamine drugs chlorcyclizine and diphenhydramine. 2. Glucose reverses the blocking action of toluidine blue. Calcium does not. 3. In the guinea-pig mesentery, mast cell damage induced by promethazine is inhibited by toluidine blue. Glucose does not reverse this blocking action. Damage by compound 48/80 is not inhibited. 4. Two other phenothiazine stains—methylene blue and thionin—also inhibit rat mesentery mast cell damage by compound 48/80. For its inhibiting action thionin requires a much lower concentration than the other two dyes. 5. The action of methylene blue and thionin on rat mast cells is influenced by the pH of the incubation medium. Much higher concentrations of these stains are needed to protect mast cells from the action of compound 48/80 when the pH is lowered from 8.4 to 7.0. Toluidine blue, however, is not influenced by pH changes. 6. “In vitro” staining of mast cells with methylene blue and thionin is also influenced by pH. At 8.4 the cells are well stained, while at 7.0 they are barely stained at all. 7. It is concluded that phenothiazine stains block mast cell damage by inhibiting cell metabolism. Therefore, besides its lytic action, antihistamines must also act through an energy-requiring process similar to that of compound 48/80. In the guinea-pig, promethazine damages mast cells through a mechanism different from that of compound 48/80, although both are non energy-requiring. Either in the rat or in the guinea-pig the phenothiazine stains do not seem to inhibit the action of mast cell damaging agents by blocking their access to the active cell sites.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00503981
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Presence of Locusta diuretic hormone in endocrine cells of the ampullae of locust Malpighian tubules (1996)
    Springer
    Cell & tissue research 285 (1996), S. 331-339 
    Details
    ISSN: 1432-0878
    Keywords: Key words: Diuretic hormone ; Endocrine cells ; Midgut ; Malpighian tubules ; Bioassay ; Immunocytochemistry ; Locusta migratoria (Insecta)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. This is an investigation of an endocrine cell type in the midgut of the migratory locust Locusta migratoria. This cell type is found in the posterior region of the midgut and is especially common in the ampullae through which Malpighian tubules drain into the gut at the midgut-hindgut junction. Strong Locusta diuretic hormone-like immunoreactivity in these cells was colocalized with FMRFamide- and substance P-like immunoreactivities. At the ultrastructural level, immunoreactivity for Locusta diuretic hormone was found in spherical granules (mean diameter of 450 nm), the contents of which showed variable electron density. Fractionation of a methanolic extract of the ampullae by reversed-phase high performance liquid chromatography revealed the presence of two peaks of Locusta diuretic hormone-like immunoreactive material, both of which stimulate cyclic AMP production by isolated Malpighian tubules. The more hydrophobic material is most likely Locusta diuretic hormone, which has the same retention time when chromatographed under identical conditions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004410050650
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    Paper (German National Licenses)
    Fulltext
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