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  • 1
    Electronic Resource
    Electronic Resource
    Cell surface receptors for endogenous mouse type C viral glycoproteins and epidermal growth factor: Tissue distribution in vivo and possible participation in specific cell-cell interaction (1980)
    New York, N.Y. : Wiley-Blackwell
    Journal of Supramolecular Structure 14 (1980), S. 343-352 
    Details
    ISSN: 0091-7419
    Keywords: cell surface receptors ; type C viral glycoproteins ; growth factors ; Life Sciences ; Molecular Cell Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: We have described previously the detection and tissue distribution of free cell surface receptors for ecotropic R-MuLV envelope glycoprotein and the growth factor EGF in vivo [1]. More recently, we have reported the chromosomal map position of the ecotropic viral receptor and its conservation between subspecies of the genus Mus [2]. This work has shown, for the first time, the presence of multiple, independently segregating cell surface receptor genes specific for different classes of ecotropic type C viral envelope glycoprotein. In this report we extend these findings and identify chromosome 2 as coding for the receptor used by M813, an ecotropic MuLV from a feral Asian mouse. This new receptor is probably also used by oncogenic, recombinant (MCF class) MuLV of C3H origin.
    Additional Material: 5 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jss.400140308
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Protein concentration by precipitation with pyrogallol red prior to electrophoresis (1995)
    Weinheim : Wiley-Blackwell
    Electrophoresis 16 (1995), S. 28-31 
    Details
    ISSN: 0173-0835
    Keywords: Protein concentration ; Pyrogallol red ; Urinary proteins ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: The pyrogallol red protein assay (Clinical Chemistry 1986, 32, 1551-1554) is based upon formation of a blue protein-dye complex in the presence of molybdate under acidic conditions. However, centrifugation of the assay mixture results in loss of color yield and precipitation of the protein-dye complex which can be recovered and resolubilized to achieve protein concentration prior to sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The method has been evaluated relative to trichloroacetic acid (TCA) precipitation for recovery and electrophoresis of commercial protein and peptide molecular weight markers. Precipitation with pyrogallol red-molybdate (PRM) gives better and more uniform recovery of both proteins and peptides as compared to TCA. The lower limit of PRM precipitation is similar to TCA and corresponds to 1 μg protein per mL assay mixture. This is equivalent to 100 μL of 10 μg/mL protein using the standard protein assay or 1 μg/mL protein using a modified assay incorporating a fivefold concentrate of the dye reagent. Application of the method is demonstrated by concentration of urinary proteins. The method is simple and economic and useful for conserving trace amounts of precious sample as it allows recovery of protein for electrophoresis following protein assay.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/elps.1150160107
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Two-dimensional electrophoresis of human urinary proteins following concentration by dye precipitation (1996)
    Weinheim : Wiley-Blackwell
    Electrophoresis 17 (1996), S. 1265-1272 
    Details
    ISSN: 0173-0835
    Keywords: Coomassie Brilliant Blue ; Pyrogallol red ; Silver staining ; Two-dimensional electrophoresis ; Urinary proteins ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: The use of dye precipitation for concentration of proteins prior to 2-DE has been demonstrated by application to human urine. The precipitation methods (T. Marshall and K. M. Williams, Clin. Chem. 1993, 39, 2314-2318; T. Marshall et al., Electrophoresis 1995, 16, 28-31) have been adapted for two-dimensional electrophoresis (2-DE) by modifying the reagent composition and adding sodium dodecyl sulphate (SDS) to the assay mixture. These modifications extend the precipitation range of the methods and overcome the problem of soluble glycoprotein-dye complexes. The protein-dye complex is recovered by centrifugation and dissolved in a small volume of sample denaturing buffer. The dye separates from the protein on 2-DE (forming a sharp band at the anodal end of the isoelectric focusing gel) so that the positional coordinates of the polypeptides are unaffected by the treatment. Dye precipitation provides a simple, rapid and highly economic method for concentrating urines of low to intermediate protein content (0.02-0.50 g/L) prior to 2-DE analysis.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/elps.1150170716
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    Paper (German National Licenses)
    Fulltext
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