ISSN:
0570-0833
Schlagwort(e):
combinatorial chemistry
;
molecular recognition
;
NMR spectroscopy
;
proteins
;
Combinatorial chemistry
;
Molecular recognition
;
NMR spectroscopy
;
Proteins
;
Chemistry
;
General Chemistry
Quelle:
Wiley InterScience Backfile Collection 1832-2000
Thema:
Chemie und Pharmazie
Notizen:
Combinatorial chemistry is a laboratory emulation of natural recombination and selection processes. Strategies in this developing discipline involve the generation of diverse, molecular libraries through combinatorial synthesis and the selection of compounds that possess a desired property. Such approaches can facilitate the identification of ligands that bind to biological receptors, promoting our chemical understanding of cellular processes. This article illustrates that the coupling of combinatorial synthesis, multidimensional NMR spectroscopy, and biochemical methods has enhanced our understanding of a protein receptor used commonly in signal transduction, the Src Homology 3 (SH3) domain. This novel approach to studying molecular recognition has revealed a set of rules that govern SH3-ligand interactions, allowing models of receptor-ligand complexes to be constructed with only a knowledge of the polypeptide sequences. Combining combinatorial synthesis with structural methods provides a powerful new approach to understanding how proteins bind their ligands in general.
Zusätzliches Material:
20 Ill.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1002/anie.199509531
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