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Monoclonal antibody TOP 71 recognizes a tonoplast protein of wide distribution in the plant kingdom (1993)

Liedtke, C. ; Martiny-Baron, G. ; Volkmann, D. ; [et al.]

Springer

Planta 190 (1993), S. 491-497

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ISSN: 1432-2048

Keywords: Lepidium ; Membrane protein ; Monoclonal antibody TOP 71 ; Plasma membrane ; Tonoplast

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract Highly purified tonoplast and plasma-membrane vesicles isolated from roots of Lepidium sativum L. (garden cress) were used as a starting material for generating a monoclonal antibody against plant tonoplast. Tonoplast vesicles were isolated by discontinuous-sucrose-gradient centrifugation followed by free-flow electrophoresis. The deglycosylated tonoplast fraction was used to generate monoclonal antibodies by immunization of Balb/c-mice and by fusion of their β-lymphocytes with the mouse cell line X 63 Ag 8.653. Using plasma membrane purified by two-phase partitioning and freeflow electrophoresis to define the negative signal in screening, and purified tonoplast to define the positive signal in screening, a monoclonal antibody (TOP 71) was obtained which recognized a tonoplast protein of 71 kDa by immunoblotting in cress-root membrane fractions. Two-dimensional gel electrophoresis, affinoblotting and binding to concanavalin A showed that the TOP 71-antigen was a glycosylated protein and had an isoelectric point (pI) of 4.5. The TOP 71-antigen was found in the different tissues of organs of several higher plants (Glycine max L., Curcurbita pepo L., Zea mays L.) where it did not cross-react with the purified plasma-membrane fractions of these plants. Additionally, TOP 71 recognized its antigen in microsomal fractions of two lower plants (Chara globularis Thuili., Matteucia struthiopteris Tod.).

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00224788

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Morphology of Trichoderma reesei QM 9414 in submerged cultures (1995)

Lejeune, R. ; Nielsen, J. ; Baron, G. V.

New York, NY [u.a.] : Wiley-Blackwell

Biotechnology and Bioengineering 47 (1995), S. 609-615

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ISSN: 0006-3592

Keywords: Trichoderma reesei ; image analysis ; morphology ; modeling ; Chemistry ; Biochemistry and Biotechnology

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology

Notes: The microscopic morphology of Trichoderma reesei QM 9414, growing in submerged culture, was studied by image analysis. The morphology was characterized by the total hyphal length, the total number of tips, the number of actively growing tips, and the length of the main hypha. To describe the growth of a single mycelium a simple model is set-up. The main features of the model are: (1) saturation type kinetics for the tip extension of the individual branches within the mycelium; and (2) random branching with a frequency function, which is proportional to the total hyphal length. The model is used to simulate a population of mycelia, where spore germination is described with a log-normal distribution. From the simulation of the population, the average properties of the mycelia, e.g., the average total hyphal length, are calculated, and by fitting the model to experimental data the model parameters are estimated. Finally, the distribution function with respect to the mycelia properties, that is, number of tips and total hyphal length, is calculated, and it corresponds well with the experimental determination of the distribution function. © 1995 John Wiley & Sons Inc.

Additional Material: 8 Ill.