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  • 1
    Electronic Resource
    Electronic Resource
    Separation of non-proliferating from proliferating thymic lymphocytes based on light scatter analysis (1979)
    Springer
    Cell & tissue research 200 (1979), S. 443-451 
    Details
    ISSN: 1432-0878
    Keywords: Light scatter sorting ; Thymus ; Cell cycle ; Morphology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Two populations of thymic lymphocytes from eight-week-old mice were separated based on the light scatter intensity they generated while passing through a flow cell analyser. The two populations were subsequently studied morphologically and analysed for the relative amount of DNA present in each cell. Cells having a low light scatter intensity had a high nuclear: cytoplasmic ratio, a heterochromatic nucleus, and a proliferative index (S + G2 + M) of only 23%. Cells with a high light scatter intensity had a low nuclear: cytoplasmic ratio, dispersed chromatin, frequently a uropod with budding microvilli and a proliferative index of 71%. The low light scatter cells resemble small cortical thymocytes or lymphocytes from the thoracic duct described by others. The highest light scatter cells resemble medium lymphocytes shown by others to be proliferative.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00234855
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    An in vitro morphological study of the mouse visceral yolk sac and possible yolk sac immunocyte precursors (1977)
    Springer
    Cell & tissue research 184 (1977), S. 113-119 
    Details
    ISSN: 1432-0878
    Keywords: Mouse yolk sac ; Immunocyte precursors ; Organ culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Mouse visceral yolk sac has been organ cultured from 9 days of gestation, a time prior to the thymus being lymphoid, until 12 days of gestation, a time after which the thymus is lymphoid. During the culture period the endodermal epithelial cells survived well, erythropoiesis diminished, endothelial-lined cavities formed in the mesodermal mass, and cells developed which have been classified as large, medium and small immunocyte precursors. The cytoplasm of the immunocyte precursors contains polysomes, spherical mitochondria, a few profiles of rough endoplasmic reticulum, occasional granules and a large Golgi complex. This study offers morphological support for the yolk sac origin of immunocyte precursors in the mouse which may seed the thymus and liver.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00220531
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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