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  • Mutant  (2)
  • Quantitative genetics  (2)
  • 1
    Electronic Resource
    Electronic Resource
    Theory for identification of marker locus-QTL associations in population by line crosses (1992)
    Springer
    Theoretical and applied genetics 85 (1992), S. 101-104 
    Details
    ISSN: 1432-2242
    Keywords: Marker assisted selection ; RFLP ; QTL ; Quantitative genetics ; Corn breeding
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The objective of this paper is to present genetic theory demonstrating the conditions under which it should be possible to identify molecular marker-quantitative trait locus (QTL) associations in crosses of random-mating populations to inbreds. Using as an example the cross of a corn (Zea mays L.) population to an inbred, the expected disequilibrium for testcross and per se performance of F2, F3, BC1 (to the inbred) and recombinant inbred generations was derived for cases where a marker allele is linked to an unfavorable QTL allele in the inbred and where the marker allele is linked to a favorable QTL allele in the inbred. Disequilibrium in segregating generations was shown to be a function of disequilibrium in the parent population, the frequency of marker and QTL alleles in the parent population, and the recombination distance between the marker and the QTL. To maximize the opportunity to identify a favorable QTL the following procedures are suggested: (1) Select marker loci with alleles in the parent population which are not present in the inbred. (2) Select populations known to have favorable QTL alleles not present in the inbred. (3) Use as many marker loci as possible to enhance the probability of tight linkage between the marker and the QTL.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00223850
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Rice protein mutant expressed in liquid suspension cultures: chitinases, β-glucanases and other proteins (1992)
    Springer
    Theoretical and applied genetics 84 (1992), S. 26-32 
    Details
    ISSN: 1432-2242
    Keywords: Lysine ; Rice ; Mutant ; Protein ; β-glucanases
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A rice mutant with unique protein expression/ transport properties has been established as cells in liquid suspension and partially characterized. Mutants were originally recovered from anther calli grown for three cycles at inhibitory levels of lysine + threonine and one cycle of S-(2-aminoethyl)cysteine. Cell suspension cultures were started from high lysine-containing seeds regenerated from the inhibitor selections. Cultures of the mutant produce 2 times as much protein per unit weight as is produced by the control. Significant portions of the proteins are exported from the cells into the surrounding medium. The mutant also has 20% greater lysine content in the exported protein than the control. This cell suspension line should be particularly useful for biochemical and molecular studies on protein synthesis and processing phenomena in cereals.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00223977
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Linkage disequilibrium in crosses between Illinois maize strains divergently selected for protein percentage (1994)
    Springer
    Theoretical and applied genetics 87 (1994), S. 1016-1020 
    Details
    ISSN: 1432-2242
    Keywords: Maize ; Long-term selection ; Quantitative genetics ; Corn breeding ; Protein
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The objectives of this study were two fold: (1) to determine whether divergent selection for kernel protein concentration, which produced the Illinois high protein (IHP), Illinois low protein (ILP), reverse low protein (RLP), and reverse high protein (RHP) maize (Zea mays L.) strains, had generated coupling-phase linkages among genes controlling protein concentration or other traits and (2) to measure the effectiveness of random mating in reducing linkage disequilibrium in segregating generations from crosses between the strains. To achieve these objectives, design III progenies from the F2 and F6 (produced by random mating the F2) from the crosses of IHP × ILP, IHP × RHP, ILP × RLP, and RHP × RLP were evaluated. Estimates of additive variance for percent protein in the crosses of IHP × ILP and ILP × RLP were significantly less in the F6 than in the F2 indicating the presence of coupling-phase linkages in the parents and their breakup by random mating. In addition, a significant reduction in dominance variance for grain yield from the F2 to the F6 in IHP × ILP suggested the presence of repulsion-phase linkages. No other evidence of coupling or repulsion-phase linkages was found for any of the traits measured. These results demonstrate the effectiveness of long-term divergent selection in the development of coupling-phase linkages and of random mating to dissipate linkage disequilibrium.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00225797
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Export of β-1,3-glucanase from mutant rice cells rechallenged and stressed with lysine plus threonine (1996)
    Springer
    Theoretical and applied genetics 92 (1996), S. 255-262 
    Details
    ISSN: 1432-2242
    Keywords: Rice ; β-1,3 glucanases ; Mutant ; Protein export ; Extracellular ; Cell culture ; Stress ; Pathogenesis-related
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Mutant rice cells (Oryza sativa L.) grown in liquid suspension cultures exported greater quantities of protein and β-glucanases than controls. These mutants were isolated from anther calli resistant to 1 mM lysine plus threonine (LT), regenerated and reestablished as cell suspension cultures from seeds. Cellular protein levels are genetically conditioned, and the levels of extracellular proteins and enzyme activities are inversely related to that of the cellular portions. The rechallenge of cells with 1 mM LT inhibited the expression of both β-1,3-glucanases and β-1,4-glucosidases but had no significant effect upon the levels of chitinase activity. Mutant cells were more sensitive than controls to stress caused by exogenous LT. In general, under exogenous LT stress the mutant/control ratio for extracellular glucanases increased as the assay conditions were changed from a basic to an acidic pH. The specific activity of βglucanases was highest in media and lowest in cells. Both the mutant and control cells exported β-glucanases into the suspension medium, but the level of activity in media was greater in that in which the mutant was suspended. The export was probably modulated by the internal protein levels which were highest in mutant cells without LT. Seedlings from mutants with enhanced lysine also had enhanced acidic β-glucanase activity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00223382
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    Paper (German National Licenses)
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