ISSN:
1617-4623
Keywords:
Cap site
;
Mutator
;
Post-transcriptional regulation
;
Shrunken
;
Transcription
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Insertion of a Mu transposable element at the Shrunken 1 (Sh1) locus of maize has resulted in kernels with the typical collapsed appearance of sh mutants. Molecular analysis of the mutant gene has revealed the presence of a 1.4 kb insertion immediately upstream from the normal transcriptional start site. Mu insertion has brought about a series of changes in gene expression: the mRNA cap site has been shifted downstream so that it now lies inside the Mu element; transcription is reduced approximately sixfold. and the sh mRNA steady-state level is less than 4% of that found in the nonmutant. This disparity reflects a mutational defect in post-transcriptional regulation which is manifested as a decrease in Sh RNA abundance.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00340191
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