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  • 1
    Electronic Resource
    Electronic Resource
    Organization of the ribosomal RNA genes in Mycoplasma hyopneumoniae: The 5S rRNA gene is separated from the 16S and 23S rRNA genes (1986)
    Springer
    Molecular genetics and genomics 205 (1986), S. 428-433 
    Details
    ISSN: 1617-4623
    Keywords: Mycoplasma hyopneumoniae ; Ribosomal RNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary In order to study the organization of the ribosomal RNA genes of Mycoplasma hyopneumoniae the rRNA genes were cloned in phage vectors λEMBL3 and λEMBL4. By subcloning the restriction fragments into various plasmids and analysing the resulting clones by Southern and Northern blot hybridization, a restriction map of the rRNA genes was generated and the organization of the rRNA genes was determined. The results show that the genes for the 16S and 23S rRNAs are closely spaced and occur only once in the genome, whereas the 5S rRNA gene is separated from the other two genes by more than 4 kb.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00338078
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Analysis of transcription and processing signals of the 16S–23S rRNA operon of Mycoplasma hyopneumoniae (1986)
    Springer
    Molecular genetics and genomics 205 (1986), S. 434-441 
    Details
    ISSN: 1617-4623
    Keywords: Mycoplasma hyopneumoniae ; Ribosomal RNA genes ; Promoter ; rRNA Processing
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The 16S and 23S rRNA genes of Mycoplasma hyopneumoniae are closely spaced in one operon. The two genes are separated by a spacer region of 500 bp which shown no sequence homology to bacterial tRNA genes. Within this operon seven 5′ and five 3′ ends of various rRNA species were mapped and the corresponding DNA was sequenced. The results are consistent with the following model for synthesis of rRNAs: Transcription of the operon is initiated from either of two tandemly arranged promoters leading to a large precursor RNA consisting of both 16S and 23S rRNAs. This primary transcript is first cleaved within stem structures surrounding the two rRNAs to yield premature 16S and 23S rRNAs. By further processing events the mature 5′ and 3′ ends are generated. The promoter sequences of this operon differ from those of other eubacterial promoters in lacking the typical -35 region. The putative termination site at the 3′ end of the operon is reminiscent of rho-independent terminators in Escherichia coli.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00338079
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
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