ISSN:
1573-6903
Keywords:
Myelin basic protein
;
esterase activity
;
ethylene glycol and salt
;
continuous polymer bed
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract In this paper we describe a fast and mild method based on the use of a unique cation exchanger and buffers containing ethylene glycol and salt for the purification of the myelin basic protein (MBP; MW 18.5 kDa). MBP thus purified hydrolyses catalytically p-nitrophenyl acetate. This esterase activity facilitates not only the purification of MBP but also indicates that probably it is in its native state, i.e. there is a good chance that the purified molecules are structurally and chemically identical. This is a prerequisite to obtain crystals appropriate for x-ray diffraction and other studies.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01705531
Permalink