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  • 1
    Electronic Resource
    Electronic Resource
    Tris(hydroxmethyl)aminomethane permits the expression of insulin-induced receptor loss in isolated rat adipocytes
    Amsterdam : Elsevier
    Biochemical and Biophysical Research Communications 102 (1981), S. 646-653 
    Details
    ISSN: 0006-291X
    Keywords: [abr] BSA; bovine serum albumin ; [abr] DNP; 2,4,dinitrophenol ; [abr] Hepes; 4-(2-hydroxymethyl)-1-piperazine-2-ethanosulfonic acid ; [abr] MEM; minimal essential medium ; [abr] Tricine; Tris (hydroxy-methyl)methyglycine ; [abr] Tris; Tris(hydroxymethyl)aminomethane
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/S0006-291X(81)80181-7
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    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Tris(hydroxmethyl)aminomethane permits the expression of insulin-induced receptor loss in isolated rat adipocytes
    Amsterdam : Elsevier
    Biochemical and Biophysical Research Communications 102 (1981), S. 646-653 
    Details
    ISSN: 0006-291X
    Keywords: [abr] BSA; bovine serum albumin ; [abr] DNP; 2,4,dinitrophenol ; [abr] Hepes; 4-(2-hydroxymethyl)-1-piperazine-2-ethanosulfonic acid ; [abr] MEM; minimal essential medium ; [abr] Tricine; Tris (hydroxy-methyl)methyglycine ; [abr] Tris; Tris(hydroxymethyl)aminomethane
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/S0006-291X(81)80181-7
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Abnormal regulation of cell membrane fluidity in diabetic nephropathy (1998)
    Springer
    Diabetologia 41 (1998), S. 337-342 
    Details
    ISSN: 1432-0428
    Keywords: Keywords Diabetic nephropathy ; fluidity ; anisotropy ; N-ethylmaleimide ; erythrocyte ; insulin-dependent diabetes mellitus.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary An abnormality of the physical properties of the cell membrane may underlie the defect that unites the clinical and biochemical abnormalities found in subjects with diabetic nephropathy. The cell membrane is linked both structurally and functionally with the cytoskeleton. The fluorescence anisotropy, a measure of membrane fluidity, was studied at baseline and after modulation of cytoskeletal proteins by thiol group alkylation with N-ethylmaleimide (NEM). 1,6-diphenyl-1,3,5-hexatriene (DPH) was used to assess anisotropy in the deep hydrophobic regions of the lipid bilayer and trimethylammonium-diphenylhexatriene (TMA-DPH) was used to assess the superficial, relatively hydrophilic regions. We compared 17 subjects with insulin-dependent diabetes mellitus (IDDM) and nephropathy with 17 control subjects with IDDM and 24 non-diabetic control subjects. Median TMA-DPH anisotropy (0.271 (0.239–0.332) vs 0.269 (0.258–0.281) vs 0.275 (0.246–0.287)) and DPH anisotropy (0.221 (0.193–0.261) vs 0.227 (0.197–0.253) vs 0.226 (0.193–0.245)) were similar in erythrocytes from the three groups. However after alkylation of protein thiol groups with NEM clear differences emerged. In the control subjects with and without IDDM there was a significant fall in TMA-DPH anisotropy compared to the subjects with diabetic nephropathy in whom the addition of NEM had no effect (ΔTMA-DPH anisotropy –0.005 (–0.020– + 0.006) vs –0.005 (–0.011– + 0.016) vs + 0.002 (–0.010 – + 0.008) p 〈 0.001). This finding was confirmed when the deep regions of the lipid bilayer were assessed using DPH (ΔDPH anisotropy –0.017 (–0.029 –– 0.007.) vs –0.015 (–0.029 – + 0.001) vs + 0.003 (–0.021 – + 0.018) p 〈 0.001). We conclude that cytoskeletal modulation of the physical properties of the cell membrane lipids by proteins is abnormal in subjects with diabetic nephropathy. Such an abnormality could explain some of the clinical and metabolic abnormalities found in this condition. [Diabetologia (1998) 41: 337–342]
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001250050912
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Thiol group modulation of sodium-lithium countertransport kinetics in diabetic nephropathy (1997)
    Springer
    Diabetologia 40 (1997), S. 1079-1084 
    Details
    ISSN: 1432-0428
    Keywords: Keywords Diabetic nephropathy ; sodium-lithium countertransport ; thiol group ; N-ethylmaleimide ; erythrocyte ; insulin-dependent diabetes mellitus.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Abnormal erythrocyte sodium-lithium countertransport (Na-Li CT) activity, traditionally measured at a single sodium concentration of 140 mmol · l–1 (V140), may represent an inherited risk marker for diabetic nephropathy. The membrane defect underlying this association is poorly understood, though modulation by key protein thiol groups appears to be important in essential hypertension. To improve understanding of this abnormality, Na-Li CT kinetics in untreated erythrocytes and after thiol group alkylation with N-ethylmaleimide were investigated in 18 subjects with diabetic nephropathy, 20 normoalbuminuric insulin-dependent diabetic (IDDM) subjects and 18 non-diabetic individuals. Using the traditional assay, V140 was similar in subjects with diabetic nephropathy compared to IDDM control subjects vs 0.311 (0.152–0.475) (0.247 (0.111–0.498) mmol Li · h–1· l erythrocytes–1). Kinetic parameters were abnormal in subjects with diabetic nephropathy compared with diabetic and non-diabetic control subjects, with both Vmax (maximal Na-Li CT activity) (0.454 (0.257–0.963) vs 0.338 (0.183–0.972) vs 0.332 (0.213–0.603) mmol Li · h–1· l erythrocytes–1, p 〈 0.05), and Vmax/Km(So) ratio, reflecting ion association (6.03 (2.3–9.6) vs 4.73 (2.0–10.4) vs 4.48 (1.5–7.1), p 〈 0.01), significantly higher. N-ethylmaleimide decreased Km(So) and Vmax abolishing differences in Vmax/Km(So) ratio between groups (2.45 (1.18–4.21) vs 2.23 (0.96–4.3) vs 2.44 (1.4–3.7), but enhancing the differences in Vmax (0.186 (0.090–0.315) vs 0.120 (0.051–0.256) vs 0.128 (0.080–0.206) mmol Li · h–1· l erythrocytes–1, p 〈 0.0001). Of subjects with diabetic nephropathy, 78 % were outside the 75th percentile of the non-diabetic control subjects when Vmax and Vmax/Km(So) ratio were combined, compared to 20 % of the normoalbuminuric control subjects. We conclude that the traditional assay, V140, is poor at detecting individuals with diabetic nephropathy. Study of the kinetic parameters of the transporter, including thiol group modulation, suggests that increased ion association, Vmax/Km(So) ratio may represent the inherited defect and improves identification of subjects with diabetic nephropathy. [Diabetologia (1997) 40: 1079–1084]
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001250050790
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    Paper (German National Licenses)
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