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  • 1
    Electronic Resource
    Electronic Resource
    Extrinsic fluorescence probe study of human serum albumin using Nile red (1996)
    Springer
    Journal of fluorescence 6 (1996), S. 23-32 
    Details
    ISSN: 1573-4994
    Keywords: Nile red ; human serum albumin ; fluorescence spectroscopy ; protein dynamics ; protein structure ; protein unfolding
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract Nile red bound to human serum albumin (HSA) shows an order of magnitude increase in the probe's fluorescence intensity. Here, we report on the fluorescence characteristics of the probe-protein complex in Trizma buffer (pH 7.1), urea, guanidine hydrochloride, and AOT/isooctane/buffer reverse micelles using both steady—state and time-resolved fluorescence techniques. With a view to illustrating the use of extrinsic probe fluorescence spectroscopy in protein research, we demonstrate that protein unfolding can be observed through measurements of the probe's time-resolved anisotropy and steady-state fluorescence spectrum. Moreover, this shows that thermal unfolding is fundamentally different from using denaturant, with respect to changes in both the nanosecond diffusional rotation of the probe at intermediate stages and in the denatured protein's structure. Also, the large Stokes shift of Nile red allows the changes in the environment of the probe-protein complex in reverse micelles of varying waterpool size to be easily identified in the steady-state fluorescence. This was not seen in earlier work exploiting the intrinsic tryptophan fluorescence of HSA and further demonstrates the complementary information that extrinsic fluorescence probe studies can offer protein science. We discuss the complex acrylamide quenching characteristics of Nile red bound to HSA in terms of the possibility of at least two binding sites for the probe and the effect of acrylamide on the probe-protein structure at very high quencher concentrations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00726723
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Engineering mechanics and materials research in the information technology age (1999)
    Springer
    Applied mathematics and mechanics 20 (1999), S. 1305-1309 
    Details
    ISSN: 1573-2754
    Keywords: engineering mechanics ; materials ; information technology ; NSF ; N13 ; TB12 ; TB39
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Mathematics , Physics
    Notes: Abstract In this paper, importance of information technology research and development for economic growth and prosperity is presented. Major missio of the research for fundamental science and engineering base is discussed. Critical points of the mechanics and materials research in the 21 st Century are proposed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02459162
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    Paper (German National Licenses)
    Fulltext
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