ISSN:
1432-203X
Keywords:
Key words Mature and immature embryos
;
Narcissus confusus
;
Galanthamine
;
Amaryllidaceae alkaloids
;
Callus
;
Bulblet
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract In vitro cultures from two strains of Narcissus confusus (Amaryllidaceae) initiated from mature seeds were screened for their ability to produce alkaloids. Protocols for callus induction, somatic embryogenesis and organogenesis were established. The alkaloid contents were determined by HPLC. Undifferentiated calli produced small amounts of galanthamine, which increased with the degree of tissue differentiation. Scanning electron micrographs of the cultures at different stages of development were made. Embryogenic friable calli were maintained in culture for more than 2 years, retaining a high regeneration capability. All regenerated plants showed normal morphological characteristics.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s002990050636
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