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pUB307 mobilizes resistance plasmids from Escherichia coli into Neisseria gonorrhoeae (1988)

Piffaretti, Jean-Claude ; Arini, Achille ; Frey, Joachim

Springer

Molecular genetics and genomics 212 (1988), S. 215-218

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ISSN: 1617-4623

Keywords: Neisseria gonorrhoeae ; IncP plasmids ; Mobilization ; Conjugation ; Cloning vector

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Summary The plasmid pUB307, a derivative of RP1, is a conjugative, broad-host-range plasmid. We have shown that this element mobilizes gonococcal resistance plasmids from Escherichia coli to Neisseria gonorrhoeae, thus providing evidence that extrachromosomal elements can efficiently enter gonococci by conjugation. Furthermore, pUB307 can also be used as a helper element to mobilize the cloning vector pLES2 into N. gonorrhoeae. This finding significantly increases the usefulness of pLES2 as a shuttle vector between E. coli and gonococcus.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00334687

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