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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Pflügers Archiv 322 (1971), S. 264-277 
    ISSN: 1432-2013
    Keywords: Retina ; Sepia officinalis ; Ionic Movements ; Ouabain ; Rubidium ; Netzhaut ; Sepia officinalis ; Ionenbewegungen ; Ouabain ; Rubidium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The intracellular cation concentrations in isolated Sepia retina were obtained by flamephotometric cation determinations before and after incubation in artificial seawater with choline replacing sodium. Intracellular Na and K concentrations of 340 and 145 mmole/l water respectively were found against extracellular Na and K concentrations of 470 mmol/le and 10 mmole/l. The influx kinetics of86Rb shows that rubidium is taken up against its concentration gradient. Ouabain (10−4–10−3M) markedly inhibits this uptake. The efflux kinetics revealed a fastexchanging extracellular compartment (t 1/2=1.5 min) and a slowly-exchanging intracellular compartment (t 1/2=34 min). A doubling of the efflux rate by ouabain was explained partly in terms of cell depolarization, and partly either as a small direct increase in permeability or inhibition of back-pumping from the extracellular space.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Pflügers Archiv 322 (1971), S. 278-286 
    ISSN: 1432-2013
    Keywords: Retina ; Sepia officinalis ; Illumination ; Ouabain ; Retinaldehyde ; Rubidium ; Netzhaut ; Sepia officinalis ; Beleuchtung ; Ouabain ; Retinaldehyde ; Rubidium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The86Rb efflux from isolated Sepia retina incubated in artificial sea water was raised 1.7–4.8 fold upon illumination with light of intensities ranging from 5×1012 to 5.6×1015 effective photons/cm2/sec. There was a simultaneous fall of 6% in the rubidium content. Addition of retinaldehyde (10−4M) or dinitrofluorobenzene (2×10−3 M) gave 45% and 35% increases in the dark efflux rate. Retinol (10−4 M) gave a smaller effect and retinoic acid (10−4 M) was without effect. The presence of ouabain (10−4 M) reduced the effect of light on the efflux rate. This effect could be explained by the decreased rubidium concentration gradient across the membrane.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
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