ISSN:
1432-0533
Keywords:
Glial fibrillary acidic protein
;
Neuron-specific enolase
;
Monoclonal antibodies
;
Immunocytochemistry
;
nervous system tumors
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary A large series of central and peripheral nervous system tumors was studied for the presence of glial fibrillary acidic protein (GFAP) and γ-enolase (neuron-specific enolase, NSE), using specific monoclonal antibodies (mAbs). Occurrence in and specificity of GFAP to glial and mixed tumors was confirmed and depended on the malignancy grade and features such as meningeal invasion. Using a well-characterized mAb, γ-enolase was demonstrated in neuronal, as well as in a whole range of non-neuronal tumors. This lack of specificity of γ-enolase prohibits its use as an exclusive neuronal marker. Nevertheless quantization or comparison with other types of enolases could still prove to be useful in well-defined situations. The advantages inherent to mAbs and a highly sensitive detection system turn GFAP stainings into a specific and readily reproducible technique.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00687791
Permalink
