ISSN:
1573-6903
Keywords:
Neuronal cultures
;
glial cultures
;
protein kinase C
;
phorbol ester receptor
;
spontaneously hypertensive rat
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract Binding of [3H]PDB has been measured in the present study to determine the levels of protein kinase C in the neuronal and astrocytic glial cells in culture from rat brain. Binding of [3H]PDB to homogenates of cultured neuronal cells from the brains of normotensive and hypertensive rats was time-dependent and specific. The relative potency for competition by various phorbol esters to [3H]PDB binding was TPA 〉 β-PDD 〉 POE 〉 α-PDD ≥4αphorbol. Scatchard analysis showed that neuronal cultures from normotensive rat brains contained 2–3 fold more phorbol ester receptors compared with the glial cultures from the same brains. No differences in theK d andB max were observed between neuronal cultures from normotensive and spontaneously hypertensive rat brains. These studies suggest that the phorbol ester receptors are primarily localized in neuronal cells.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00971854
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