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  • 1
    Electronic Resource
    Electronic Resource
    Hydrogen-stimulated CO2 fixation and coordinate induction of hydrogenase and ribulosebiphosphate carboxylase in a H2-uptake positive strain of Rhizobium japonicum (1979)
    Springer
    Archives of microbiology 123 (1979), S. 1-8 
    Details
    ISSN: 1432-072X
    Keywords: Hydrogenase ; Ribulosebisphosphate carboxylase ; Hydrogen uptake ; Rhizobium japonicum, CO2 fixation ; Propionyl coenzyme A carboxylase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract H2-uptake positive strains (122 DES and SR) and H2-uptake negative strains SR2 and SR3 of Rhizobium japonicum were examined for ribulosebisphosphate (RuBP) carboxylase and H2-uptake activities during growth conditions which induced formation of the hydrogenase system. The rate of 14CO2 uptake by hydrogenase-derepressed cells was about 6-times greater in the presence than in the absence of H2. RuBP carboxylase activity was observed in free-living R. japonicum strains 122 DES or SR only when the cells were derepressed for their hydrogenase system. Hydrogenase and RuBP carboxylase activities were coordinately induced by H2 and both were repressed by added succinate. Hydrogenase-negative mutant strains SR2 and SR3 derived from R. japonicum SR showed no detecyable RuBP carboxylase activities under hydrogenase derepression conditions. No detectable RuBP carboxylase was observed in bacteroids formed by H2-uptake positive strains R. japonicum 122 DES or SR. Propionyl CoA carboxylase activity was consistently observed in extracts of cells from free-living cultures of R. japonicum but activity was not appreciably influenced by the addition of H2. Neither phosphoenolpyruvate carboxylase nor phosphoenolpyruvate carboxykinase activity was detected in extracts of R. japonicum.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00403496
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  • 2
    Electronic Resource
    Electronic Resource
    Mutants of Rhizobium japonicum defective in nodulation (1982)
    Springer
    Archives of microbiology 132 (1982), S. 219-224 
    Details
    ISSN: 1432-072X
    Keywords: Rhizobium ; Nitrogen fixation ; Nodules ; Soybean
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Several mutants defective in nodulation were isolated from Rhizobium japonicum strains 3I1b110 and 61A 76. Mutants of class I do not form nodules after incubation with soybean [Glycine max (L.) Merrill] for 17 days, but will do so by 28 days. When host plants other than G. max are infected with several of these strains, there is no detectable difference in the time of nodulation or size of nodules as compared to the wild type. Two mutants of class I (i. e., SM1 and SM2) have been shown previously to be altered in the lipopolysaccharide portion of their cell wall. Mutants of class II are not slow to nodulate but form fewer nodules than the wild type on all the host plants tested. Mutants of class III are unable to form nodules. Some bacteriophage-resistant mutants, altered in cell surface structure, fall into this class. Two mutants of class III do not bind to soybean roots.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00407954
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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