ISSN:
1432-072X
Keywords:
Neurospora crassa
;
Macroconidiation
;
Microcycle
;
Heat-shock
;
Differential DNA synthesis
;
Nuclei
;
Inhibitors of DNA synthesis
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract In conidia of Neurospora crassa germinating at 25°C, DNA synthesis measured by incorporation of tritiated adenosine reaches a maximum soon after the outgrowth of the germ tube (6–7h after inoculation). In conidia heat-treated at 46°C (for 15h), a maximum of incorporation of the DNA precursor occurs already 1h after inoculation, then the incorporation progressively declines until the end of the heat-shock. When such conidia are shifted to 25°C, a maximum of DNA synthesis occurs during the development of the presumptive conidiophore as at the outgrowth of normal germ tubes. This wave of DNA synthesis is followed by a second maximum of DNA synthesis, occurring only in the microcyclized cultures, when the premature differentiation of proconidia takes place. Prevention of this second wave of DNA synthesis with hydroxyurea or 5-fluorodeoxyuridine respectively reduces or fully inhibits such induced conidial differentiation.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00405404
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