ISSN:
0749-1581
Keywords:
2D NMR
;
Solution conformation
;
Nucleic acids
;
DNA structure
;
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
Two-dimensional NMR has developed into an important tool for the study of solution structures of nucleic acids. The conformational information is generally derived from the three bond coupling constants (J) and interproton distances (r). Strategies have been developed to obtain J values using COSY and J-resolved spectroscopy. Estimation of interproton distances involves measurement of NOESY build-up curves as a function of mixing time. This information can be coupled with structural refinement techniques to obtain the three-dimensional structure of nucleic acids. Here we have described the current status of this important field with particular reference to advancements in applications to DNA structures.
Additional Material:
24 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/mrc.1260261019
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