ISSN:
1432-072X
Keywords:
Synechocystis
;
Lipopolysaccharide
;
Lipid A
;
O-Methyl sugars
;
Chemotypes
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Lipopolysaccharides were found in four strains of Synechocystis. Depending on the strain, they were extracted into either the water or into the phenol phase of phenol-water extracts. The lipopolysaccharides of all four strains contain fucose, mannose, galactose, glucose and glucosamine. l-Glycero-d-mannoheptose and 2-keto-3-deoxyoctonate are lacking. The strain-specific sugars are dominated by O-methyl sugars in three of the four strains: Synechocystis PCC 6803 contains 2,3-di-O-methyl-fucose, 2-O-methyl-fucose and 2-O-methylxylose. In Synechocystis PCC 6807 a 6-O-methylheptose and 2-O-methyl-mannose, in Synechocystis PCC 6308 2-O-methyl-mannose was identified. Lipid A, although difficult to be split off from the polysaccharide moiety, is indicated in all four strains by the presence of β-hydroxypalmitic and β-hydroxymyristic acids and of glucosamine. In addition, a branched β-hydroxypentadecanoic acid (anteiso) was found. The phosphorus content of the four lipopolysaccharides amounts to less than 0.3% of dry weight. The lipopolysaccharides from Synechocystis show O-specific activity. Their reactivity in homologous O-antisera, however, is low when tested by passive hemagglutination. In immunoelectrophoresis, no migration of lipopolysaccharide was observed.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00427196
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