ISSN:
1573-4919
Keywords:
O-haptens
;
lipopolysaccharides
;
Brucella abortus
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Chemistry and Pharmacology
,
Medicine
Notes:
Abstract The O-haptens of the major fraction (f5A) of B. abortus (Strains 2308 and 19) membrane bound smooth lipopolysaccharide (sLPS) were prepared by hydrolysis of f5A native sLPS in 1% acetic acid at 100°C for 2 h. After hydrolysis, O-haptens were separated from Lipid A-protein complex by centrifugation, and from small fragments by ultrafiltration of molecular weight cut-off (MWCO) 1.0 × 103. These carbohydrate haptens were identified by precipitin-inhibition assay and further fractionated by both membrane filtration and dialysis. The size distributions of carbohydrate haptens of endotoxins (f5A) ranged from oligosaccharides up to polysacchandes of 1.0 × 104 MWCO. Three major fractions of MWCO 8.0 − 10.0 × 103, 3.5 − 5.0 × 103 and 〈 1.0 × 103 from both strains 2308 and 19 contained more than 85% of the total immunoactive materials. These fractions of haptens were subjected to composition, proton and 13C NMR analysis and were found to be a homopolymer of α1→2 linked, 4,5-dideoxy-4-formamido-D-mannose (N-formylperosamine), which is identical to O-haptens of B. abortus strain 119.3 and Yersinia enterocolitica serotype 0:9 and similar to Vibrio cholerae 569B (INABA). Fractions of these haptens exhibited similar inhibitory reactivities in a precipitin-inhibition assay as expressed as µmoles of monosaccharide of anhydro-N-formyl perosamine. They were about 480 times as active as Me α → DMan or DMan.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00229898
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