ISSN:
1432-2013
Keywords:
Membrane properties
;
Oligodendrocytes
;
Neuroglia
;
Electrical properties
;
Potassium
;
Mouse
;
Spinal cord
;
Nervous system
;
Culture
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract The electrical properties of immunocytologically identified oligondendrocytes from embryonic mouse spinal cord maintained in culture for 3 to 6 weeks were studied by passing current and recording potential changes with two separate intracellular electrodes. The average input resistance was 3.3 M Ω and ranged from 0.7 to 16 M Ω (n=35). The input resistance increased by 19% with depolarization and decreased by 9% with hyperpolarization of 25 mV. The membrane time constant determined from the slope of the late exponential tail was 3.45±2.5 ms SD (n=15). The specific membrane resistance of three cells was determined by a simplified square pulse analysis combined with measurement of membrane area. Membrane area was estimated from photomicrographs of cells injected with Lucifer Yellow CH and stained with the cell surface-reactive antibody 04 and from electron micrographs. An average specific membrane resistance of 1.3×103 Ωcm2 and specific capacitance of 1.7 μF/cm2 were calculated. Increasing [K+]o depolarized the cells and decreased the input resistance and the time constant.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00584331
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