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  • Thylakoid membranes  (4)
  • red clover  (3)
  • Organic Chemistry  (2)
  • 1
    ISSN: 1432-2048
    Keywords: Cell cultures ; Chlorophyll protein ; Photosynthesis (light harvesting) ; Poly(A)RNA ; Thylakoid membranes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In thylakoid membranes isolated from green plants of parsley, pea, and barley, the light-harvesting chlorophyll a/b protein complex (LHCP, mol. weight: 25,000), is a major constituent. Poly(A)RNA isolated from these species was translated in a wheat germ, cell-free system. The in vitro translation products were treated with antibodies raised against the LHCP. This treatment resulted in the precipitation of a precursor protein (mol. weight: 29,000). Poly(A)RNA was also prepared from a cell culture ofPetroselinum that does not develop chloroplasts upon illumination. This poly(A)RNA is capable of stimulating amino acid incorporation in the in vitro translation system, however, it does not direct the synthesis of LHCP.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-2048
    Keywords: Chlorophyll protein ; Hordeum ; Photosynthesis (light harvesting) ; Poly(A)RNA ; Polysomes ; Thylakoid membranes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Polysomes from dark-grown and illuminated barley seedlings were translated in cell-free systems. The translation products reacting with the antibody against the light-harvesting chlorophyll a/b protein (LHCP) were analyzed by polyacrylamide gel electrophoresis. It was found that, in addition to the precursor protein of LHCP, a product was obtained that co-migrated with the mature protein. Furthermore, the results show that the light-induced proly(A)RNA for LHCP is integrated into the polysomal complex without delay, indicating that the integration of LHCP into the membrane is controlled at a higher level of gene expression.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1432-2048
    Keywords: Chlorophyll protein ; Hordeum ; Photosynthesis (light harvesting) ; Poly(A)RNA ; Polysomes ; Thylakoid membranes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Polysomes from dark-grown and illuminated barley seedlings were translated in cell-free systems. The translation products reacting with the antibody against the light-harvesting chlorophyll a/b protein (LHCP) were analyzed by polyacrylamide gel electrophoresis. It was found that, in addition to the precursor protein of LHCP, a product was obtained that co-migrated with the mature protein. Furthermore, the results show that the light-induced proly(A)RNA for LHCP is integrated into the polysomal complex without delay, indicating that the integration of LHCP into the membrane is controlled at a higher level of gene expression.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1432-2048
    Keywords: Cell cultures ; Chlorophyll protein ; Photosynthesis (light harvesting) ; Poly(A)RNA ; Thylakoid membranes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In thylakoid membranes isolated from green plants of parsley, pea, and barley, the light-harvesting chlorophyll a/b protein complex (LHCP, mol. weight: 25,000), is a major constituent. Poly(A)RNA isolated from these species was translated in a wheat germ, cell-free system. The in vitro translation products were treated with antibodies raised against the LHCP. This treatment resulted in the precipitation of a precursor protein (mol. weight: 29,000). Poly(A)RNA was also prepared from a cell culture ofPetroselinum that does not develop chloroplasts upon illumination. This poly(A)RNA is capable of stimulating amino acid incorporation in the in vitro translation system, however, it does not direct the synthesis of LHCP.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1573-5036
    Keywords: field bean ; green manure ; mesh bag ; N cycling ; N loss ; N-15 ; red clover ; subterranean clover ; timothy ; white clover
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Release of N, retention in soil, availability to a subsequent crop and total recovery of N derived from different15N-labelled plant materials decomposing in soil was investigated in two field experiments. In the first experiment five different plant species (white clover, red clover, subterranean clover, field bean and timothy) and in the second subterranean clover of different maturity (2,3 and 4 months old) were buried in mesh bags in the soil and allowed to decompose for 10 and 4 months, respectively. Most of the N released from the decaying plant materials was retained in the soil (27–46% of input). The subsequent crop (barley) took up 6–25% of input. The uptake correlated with the amount of N released from the decomposing material (r=0.936*, I experiment). Similar amounts of subterranean clover N were taken up by barley regardless to whether the material was buried in soil in the previous autumn or just before sowing of the crop. At the end of the experiments, the total recovery of the introduced plant-derived N varied between 89 and 102%. The results present evidence that the ability of the soil to retain plant-derived N is strong in comparison with the ability of the subsequent crop and different loss mechanisms to remove it.
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1573-5036
    Keywords: barley ; decomposition ; fertilization ; green manure ; liming ; N-budget ; N-release ; 15N ; red clover
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Red clover material labelled with15N was confined in mesh bags and buried into microplots in October at two field sites in Sourthern Finland. The soils (Kettula silt loam and Långvik loam) contained in the microplots were previously limed and fertilized with PK (N was given in next May) in different combinations. The following May the microplots were sown with barley and after the barley had been harvested (that August) soil samples and the mesh bags were removed. The crop, mesh bags and soil were then analysed for15N content. The soil treatments did not affect the release of clover N from the mesh bags and only slightly its retention in Kettula silt loam. The dry mass of barley obtained responded strongly to the different soil treatments. In comparison, the uptake of clover N by the barley varied much less; in Kettula silt loam a 1.6 fold increase in barley dry mass did not affect its content of clover N (12–13% of input) and in Långvik loam a ten fold increase in barley dry mass yield corresponded to a three fold increase of the content of clover N (5–16%). The total recovery of clover N (clover N in soil+mesh bags+barley harvest) was almost independent of the soil treatment; in Kettula silt loam and Långvik loam being respectively 52–56% and 71–85% of the input.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Plant and soil 105 (1988), S. 149-152 
    ISSN: 1573-5036
    Keywords: C release ; liming ; mesh bag ; Nitrogen ; N release ; red clover
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Red clover root material confined in mesh bags was buried in three different limed and unlimed soils and incubated for 196 days at room temperature. Remaining amounts of organic matter, as well as concentrations of C and N of the decomposing material were determined three times during the incubation and finally the concentration of soil mineral N and pH of remaining roots was also assessed. Liming only temporarily affected the decomposition rate of organic matter and N release, and at the end of the incubation no effects could be observed due to liming. A possible explanation is that the decomposing root residues provide a well buffered micro-environment for the decomposing microflora. Liming did not change the pH of the root residues even when 97–98% of dry mass had disappeared from the mesh bags. Concentrations of mineral N were higher in limed than in unlimed soils.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Liebigs Annalen 1989 (1989), S. 367-374 
    ISSN: 0170-2041
    Keywords: Concave base ; 1,10-Phenanthroline ; Macrobicyclic lactam ; Template cyclization ; Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Konkave Reagenzien, 2. - Makrobicyclische 1,10-PhenanthrolineDrei makrobicyclische 2,9-disubstituierte 1,10-Phenanthrolin-bislactame 4 wurden mit Gesamtausbeuten bis zu 43% synthetisiert. Dabei wurden zwei Makrocyclisierungstechniken angewandt: 1) Eine Metallionen-gesteuerte Biskondensation (Templatsynthese) eines Diamins 6 mit 1,10-Phenanthrolin-2,9-dicarbaldehyd (5) führte zu neuen makrocyclischen 1,10-Phenanthrolin-Diimin-Komplexen 7 (Schiff-Basen, als Pb2⊕-Komplexe isolierbar), die durch Reduktion mit NaBH4 in die makrocyclischen Diamine 8 übergeführt werden konnten. 2) Die makrocyclischen Diamine 8 wurden mit Dicarbonsäuredichloriden 9 nach dem Verdünnungsprinzip zu den Makrobicyclen 4 umgesetzt. - Die Synthesen der Bicyclen 4, ihre Reaktionen mit Cu⊕ und die relativen Basizitäten von 4 und Vergleichsverbindungen in Ethanol werden beschrieben.
    Notes: Three macrobicyclic 2,9-disubstituted 1,10-phenanthroline-containing bislactams 4 were synthesized in overall yields of up to 43%. Two successive macrocyclization techniques were used: i) metal ion template-assisted bis-condensations of diamines 6 with 1,10-phenanthroline-2,9-dicarbaldehyde (5), leading to new macrocyclic 1,10-phenanthroline-diimine complexes 7 (Schiff bases, isolable as their Pb2⊕ complexes), which were then reduced with NaBH4 to yield the macrocyclic diamines 8, and ii) a high dilution cyclization reaction between the macrocyclic diamines 8 and diacyl dichlorides 9. - Syntheses, complexing abilities towards Cu⊕, and relative basicities of 4 and related compounds in ethanol are reported.
    Additional Material: 2 Tab.
    Type of Medium: Electronic Resource
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  • 9
    ISSN: 0170-2041
    Keywords: Macrocycles ; Template synthesis ; Conformers ; Sulfonamides ; Pyridines ; Phenanthrolines ; Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Bimacrocyclic concave pyridinebissulfonamides 2 and concave 1,10-phenanthrolinebissulfonamides 4 have been synthesized by bridging macrocyclic pyridinediamines 12 or 1,10-phenanthrolinediamines 16 with alkylenebis(sulfonyl chloride)s 14. In contrast to concave pyridine- and 1,10-phenanthrolinebislactams 1 and 3, the new compounds 2 and 4 exhibit simpler 1H-NMR spectra and sharper melting points due to the absence of amide conformers; in comparison to 1, the basicities of 2 are decreased by a factor of ca. 100. By introduction of donor substituents into the 4-position of the pyridine ring the basicity loss can be easily compensated. The 4-diethylamino substituent increases the basicity of the concave pyridines 1 and 2 as expected (ca. + 4 pK units). Since no deviations from the expected basicity and reactivity for the amino-substituted concave pyridines 1 and 2 have been found, also in these sterically hindered pyridines, the pyridine nitrogen atom is the centre of basicity and reactivity. - The synthesis of new building blocks is described, i.e. 4-diethylamino-2,6-pyridinedicarbaldehyde (9c), the polyether-based bis(sulfonyl chloride) 14b, the monomacrocyclic diamines 12e-h with a pentaethyleneglycol chain or a 4-diethylamino substituent.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
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