ISSN:
1860-1499
Keywords:
Human ovary
;
Culture
;
Growth
;
Ovarian surface epithelium
;
Characterization
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract To isolate human ovarian surface epithelium (OSE) and studied its characteristicsin vitro, we used the scraping method with an enzymatic procedure. After incubation of the ovary in collagenase type 1 solution, the surface cells were removed by gentle scraping with surgical blade. Cells obtained as a cluster after unit gravity sedimentation with 5% bovine serum albumin in medium 199 were cultured in medium 199 containing 15% fetal bovine serum. The viable cell number in a single ovary was 0.1 to 2.7×106, and the cell population doubling time was between 7 to 10 days. Confluent monolayers were formed after 13–20 days and subcultured one to three times. The monolayers mostly had a cobblestone appearance, and fusiform or polygonal cells were also observed. By cytochemistry, immunocytochemistry and scanning and transmission electron microscopy, the cells in short-term culture were shown to have characteristics of mesothelial OSE cells.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02349671
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