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  • PACS. 27.20.+n 6 ?A? 19 – 21.10.Ft Charge distribution – 21.60.-n Nuclear structure models and methods – 25.70.-z Low and intermediate energy heavy-ion reactions  (1)
  • QTLs  (1)
  • active site  (1)
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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    The protein journal 12 (1993), S. 451-457 
    ISSN: 1573-4943
    Keywords: Carboxyl group ; Woodward's reagentK ; PEP carboxylase ; active site ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Maize leaf phosphoenolpyruvate carboxylase was completely and irreversibly inactivated by treatment with micromolar concentrations of Woodward's reagentK (WRK) for about 1 min. The inactivation followed pseudo-first-order reaction kinetics. The order of reaction with respect to WRK showed that the reagent causes formation of reversible enzyme inhibitor complex before resulting in irreversible inactivation. The loss of activity was correlated to the modification of a single carboxyl group per subunit, even though the reagent reacted with 2 carboxyl groups per protomer. Substrate PEP and PEP + Mg2+ offered substantial protection against inactivation by WRK. The modified enzyme showed a characteristic absorbance at 346 nm due to carboxyl group modification. The modified enzyme exhibited altered surface charge as seen from the elution profile on FPLC Mono Q anion exchange column. The modified enzyme was desensitized to positive and negative effectors like glucose-6-phosphate and malate. Pretreatment of PEP carboxylase with diethylpyrocarbonate prevented WRK incorporation into the enzyme, suggesting that both histidine and carboxyl groups may be closely physically related. The carboxyl groups might be involved in metal binding during catalysis by the enzyme.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1572-9788
    Keywords: genetic mapping ; genome analysis ; PCR-based markers ; physical mapping ; QTLs ; resistance genes ; synteny
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    The European physical journal 8 (2000), S. 511-520 
    ISSN: 1434-601X
    Keywords: PACS. 27.20.+n 6 ?A? 19 – 21.10.Ft Charge distribution – 21.60.-n Nuclear structure models and methods – 25.70.-z Low and intermediate energy heavy-ion reactions
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract: Loosely bound Exotic Nuclei in the neutron-halo region have very small neutron/proton separation energy implying a long tail of the last loosely bound nucleon. An accurate treatment of this tail is crucial for the description of several reaction observables. We propose, for such loosely bound Exotic Nuclei, a simple analytic expression for nucleon densities which incorporates correctly two basic physical requirements: a) The asymptotic behavior (r→∞). b) The behavior near the center (r→ 0). The expression provides, separately, the neutron and proton densities and is suitable for the description of Exotic Nuclei in the neutron-halo region. In addition it provides a simple physical insight into the density in the outer (halo) region. Glauber type calculations for reaction cross-sections, using these densities have been carried out for such nuclei. The calculated cross-sections are found to be consistent with the experimental values. The calculated densities are in good agreement with the corresponding so-called experimentally deduced densities (where available) indicating the reliability of the proposed model densities.
    Type of Medium: Electronic Resource
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