ISSN:
1573-6849
Keywords:
B1-primers
;
carcinoembryonic antigen
;
FISH
;
gene mapping
;
mouse
;
PCR
;
pregnancy-specific glycoprotein
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract PCR with primers specific for the murine B1 consensus sequence allows amplification of DNA from murine sources. We have used B1-PCR for amplifying yeast artificial chromosome (YAC) DNA which can be used to localize single YACs by fluorescencein situ hybridization. The genes for the pregnancy-specific glycoproteins Cea2 and Cea4, both belonging to the large carcinoembryonic antigen gene family, were localized by chromosomalin situ suppression hybridization of three YAC clones to murine chromosome 7A2-A3. This was facilitated by the use of the mouse lymphoma cell line WMP/WMP which contains nine pairs of Robertsonian fusion chromosomes.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00713961
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