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  • Pancreatic islets  (4)
  • beta-cell function  (2)
  • healthy volunteers  (2)
  • 1
    Electronic Resource
    Electronic Resource
    Isolated mouse pancreatic islets in culture: Effects of serum and different culture media on the insulin production of the islets (1978)
    Springer
    Diabetologia 14 (1978), S. 397-404 
    Details
    ISSN: 1432-0428
    Keywords: Pancreatic islets ; tissue culture ; calf serum ; culture media ; insulin secretion ; insulin content ; insulin biosynthesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Various conditions for tissue culture of collagenase-isolated mouse pancreatic islets were studied in an attempt to optimize the maintenance of glucose stimulated insulin biosynthesis and release in the cultured specimens. Islets which had been cultured at a physiological glucose concentration (5.5 mmol/l) in the absence of serum had an impaired glucose-stimulated insulin biosynthesis and release as well as a reduced insulin content. Thus, insulin biosynthesis was three times higher after culture in a serum supplemented medium. Further, the insulin secretion of islets cultured in the presence of serum was markedly enhanced in acute incubations with high concentrations of glucose. This response was most pronounced in islets which had been cultured free-floating. A comparison between different culture media showed that islets cultured in RPMI 1640 had the highest insulin production. The present data suggest that the most favourable conditions for long-term storage of isolated islets in culture may be obtained when the islets are maintained as free-floating explants in a culture medium consisting of RPMI 1640 supplemented with serum.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01228134
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Stimulatory effect of serum from diabetic patients on insulin release from mouse pancreatic islets maintained in tissue culture (1981)
    Springer
    Diabetologia 20 (1981), S. 60-65 
    Details
    ISSN: 1432-0428
    Keywords: Pancreatic islets ; tissue culture ; diabetic serum ; beta-cell function
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Islets of Langerhans from NMRI-mice were kept for one week in tissue culture in medium supplemented with human serum obtained from either normal healthy subjects or newly diagnosed juvenile diabetic patients before insulin treatment. Islets cultured in diabetic serum released more inslin than islets cultured in normal serum, whether tissue culture medium 199 with 5.5–8.3 mmol/1 glucose and 10% serum, or culture medium RPMI1 640 with 11 mmol/1 glucose and 0.5% serum were used. Islets kept for one week in culture with diabetic serum did not show any decrease in DNA content or glucose induced insulin secretion and biosynthesis. It is concluded that serum from newly diagnosed insulin dependent diabetic patients stimulates insulin release from isolated mouse islets kept in tissue culture. The underlying mechanism is unknown.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00253819
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Effects of L-leucine on the insulin production, oxidative metabolism and mitochondrial ultrastructure of isolated mouse pancreatic islets in tissue culture (1977)
    Springer
    Diabetologia 13 (1977), S. 59-69 
    Details
    ISSN: 1432-0428
    Keywords: Pancreatic islets ; tissue culture ; ultrastructure ; morphometry ; mitochondria ; L-leucine ; B-cell function
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary This study was performed to evaluate whether L-leucine is able to relieve the structural and functional alterations previously described in pancreatic islets exposed in vitro for a prolonged time to a subnormal glucose concentration (3.3 mM). It was found that both the impairment of secretion and the decreased rate of biosynthesis of insulin characteristic, of islets cultured for one week in 3.3 mM glucose were prevented by adding 15 mM L-leucine to the culture medium. Furthermore, the rates of tritiated water production and glucose or leucine oxidation were significantly enhanced after culture in the presence of L-leucine. The rate of DNA synthesis as estimated by the incorporation of tritiated thymidine was, however, unchanged by the presence of L-leucine in the culture medium. Leucine cultured islet cells displayed ultrastructural signs of high functional activity. A detailed morphometric examination revealed fewer but hypertrophic mitochondria. The present results suggest that L-leucine can replace glucose in several respects as a long-term stimulus of the pancreatic B-cells, possibly by acting as a metabolic substrate.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00996329
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Stimulatory effect of serum from diabetic patients on insulin release from mouse pancreatic islets maintained in tissue culture (1981)
    Springer
    Diabetologia 21 (1981), S. 60-65 
    Details
    ISSN: 1432-0428
    Keywords: Pancreatic islets ; tissue culture ; diabetic serum ; beta-cell function
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Islets of Langerhans from NMRI-mice were kept for one week in tissue culture in medium supplemented with human serum obtained from either normal healthy subjects or newly diagnosed juvenile diabetic patients before insulin treatment. Islets cultured in diabetic serum released more insu lin than islets cultured in normal serum, whether tissue culture medium 199 with 5.5–8.3 mmol/l glucose and 10% serum, or culture medium RPMI 1640 with 11 mmol/l glucose and 0.5% serum were used. Islets kept for one week in culture with diabetic serum did not show any decrease in DNA content or glucose induced insulin secretion and biosynthesis. It is concluded that serum from newly diagnosed insulin-dependent diabetic patients stimulates insulin release from isolated mouse islets kept in tissue culture. The underlying mechanism is unknown.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01789116
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
  • 5
    Electronic Resource
    Electronic Resource
    An assessment of the systemic effects of single and repeated doses of inhaled fluticasone propionate and inhaled budesonide in healthy volunteers (1996)
    Springer
    European journal of clinical pharmacology 49 (1996), S. 459-463 
    Details
    ISSN: 1432-1041
    Keywords: Key words Corticosteroids; systemic effects ; plasma cortisol suppression ; white blood cell count ; healthy volunteers
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Medicine
    Notes: Abstract The systemic effects of single and multiple doses of inhaled fluticasone propionate (FP) and budesonide were examined in 24 healthy male volunteers (age range 18–29 years). The study was of an open, placebo-controlled, randomized, three-way crossover design. On each study day, multiple blood samples were taken over a 20 h period after drug administration (after a single dose and after the last of seven doses) and area under the curve (AUC0–20) for plasma cortisol and white blood cell (WBC) counts was calculated. Results: The present study shows that multiple dosing with FP 1.0 mg b.i.d. for 3.5 days (seven doses) resulted in a marked cortisol suppression from placebo which, at 55%, was more than double that seen with a single dose (25% suppression). Multiple dosing with budesonide 0.8 mg b.i.d. resulted in a 34% suppression in plasma cortisol compared with a suppression of 26% with a single dose. The increase in systemic activity of FP after multiple dosing is confirmed by both the number of subjects with 0800 hours plasma cortisol values below normal limits and by the changes in WBC and differential counts. Conclusion: The results of the present study confirm previous findings with regard to the more marked systemic effect of FP following multiple dosing as compared with a single dose. This increase in systemic effect from single dosing to multiple dosing is significantly greater for FP than for budesonide.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002280050050
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    Paper (German National Licenses)
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  • 6
    Electronic Resource
    Electronic Resource
    An assessment of the systemic effects of single and repeated doses of inhaled fluticasone propionate and inhaled budesonide in healthy volunteers (1996)
    Springer
    European journal of clinical pharmacology 49 (1996), S. 459-463 
    Details
    ISSN: 1432-1041
    Keywords: Corticosteroids ; systemic effects ; plasma cortisol suppression ; white blood cell count ; healthy volunteers
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Medicine
    Notes: Abstract The systemic effects of single and multiple doses of inhaled fluticasone propionate (FP) and budesonide were examined in 24 healthy male volunteers (age range 18–29 years). The study was of an open, placebo-controlled, randomized, three-way crossover design. On each study day, multiple blood samples were taken over a 20 h period after drug administration (after a single dose and after the last of seven doses) and area under the curve (AUC0–20) for plasma cortisol and white blood cell (WBC) counts was calculated. Results: The present study shows that multiple dosing with FP 1.0 mg b.i.d. for 3.5 days (seven doses) resulted in a marked cortisol suppression from placebo which, at 55%, was more than double that seen with a single dose (25% suppression). Multiple dosing with budesonide 0.8 mg b.i.d. resulted in a 34% suppression in plasma cortisol compared with a suppression of 26% with a single dose. The increase in systemic activity of FP after multiple dosing is confirmed by both the number of subjects with 0800 hours plasma cortisol values below normal limits and by the changes in WBC and differential counts. Conclusion: The results of the present study confirm previous findings with regard to the more marked systemic effect of FP following multiple dosing as compared with a single dose. This increase in systemic effect from single dosing to multiple dosing is significantly greater for FP than for budesonide.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00195931
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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