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  • 1
    Electronic Resource
    Electronic Resource
    Intraspecific genetic variation in cytokinin-controlled shoot morphogenesis from tissue explants of Petunia hybrida
    Amsterdam : Elsevier
    Plant Science Letters 35 (1984), S. 237-245 
    Details
    ISSN: 0304-4211
    Keywords: Petunia ; cytokinin ; genetic variation ; shoot regeneration ; tissue culture
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/0304-4211(84)90235-9
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  • 2
    Electronic Resource
    Electronic Resource
    Cytochrome oxidase subunit II sequences in Petunia mitochondria: two intron-containing genes and an intron-less pseudogene associated with cytoplasmic male sterility (1989)
    Springer
    Current genetics 16 (1989), S. 281-291 
    Details
    ISSN: 1432-0983
    Keywords: Cytochrome oxidase subunit II ; Petunia ; Cytoplasmic male sterility ; Processed pseudogene
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The mitochondrial genome of Petunia hybrida contains two transcribed cytochrome oxidase subunit II(coxII) genes. The coding region of both genes is split by a 1.3 kb group II intron. Unlike coxII-1, which is similar to other sequenced plant coxII genes, the coxII-2 coding region is extended by 48 codons. The cytoplasmic male sterile (CMS) Petunia contains one coxII gene similar in structure and transcript pattern to the coxII-1 gene found in the fertile genome. Comparison of the sequenced coxII genes from the fertile mitochondrial genome with the coxII sequences present in the CMS-associated pcf gene from the CMS genome (Young and Hanson 1987) suggests that pcf is a processed pseudogene. A model for the generation of pcf is presented.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00422115
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  • 3
    Electronic Resource
    Electronic Resource
    Recombination between parental mitochondrial DNA following protoplast fusion can occur in a region which normally does not undergo intragenomic recombination in parental plants (1987)
    Springer
    Current genetics 12 (1987), S. 235-240 
    Details
    ISSN: 1432-0983
    Keywords: Recombination ; Somatic hybrid ; mtDNA ; Petunia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A novel restriction fragment, which was not present in either parent's mitochondrial DNA, was cloned from the mitochondrial genome of a somatic hybrid Petunia line. This fragment resulted from interspecific recombination between homologous mitochondrial DNA regions of the parental plants, Petunia line 3704 and line 3688. Hybridization with a cloned Petunia atp9 gene revealed that the regions involved in recombination carry atp9 coding sequences. Intragenomic recombination within the parental mitochondrial genomes was not detected between the atp9 regions which recombined following protoplast fusion.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00435283
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  • 4
    Electronic Resource
    Electronic Resource
    A truncated recombination repeat in the mitochondrial genome of a Petunia CMS line (1993)
    Springer
    Current genetics 23 (1993), S. 477-482 
    Details
    ISSN: 1432-0983
    Keywords: Recombination ; Petunia ; Mitochondria ; Repeat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Repeated sequences known as recombination repeats are present in the majority of plant mitochondrial genomes. Two recombination repeat sequences from Petunia have been analyzed. The two repeats are virtually identical over 1.42 kb. One of the repeats is truncated and is likely to have arisen from a rare recombination event in the full-length repeat. Two sequence-blocks within the Petunia repeat are highly similar to sequences in the 5′ flank of several plant mitochondrial genes. No sequence motifs are shared by the Petunia repeat and other sequenced plant mitochondrial recombination repeats, suggesting that the recombination occurs by an homologous, rather than a site-specific, mechanism.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00312638
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  • 5
    Electronic Resource
    Electronic Resource
    Intergenomic recombination of mitochondrial genomes in a somatic hybrid plant (1985)
    Springer
    Current genetics 9 (1985), S. 615-618 
    Details
    ISSN: 1432-0983
    Keywords: Mitochondrial DNA ; Protoplast fusion ; Petunia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Restriction site polymorphisms between two parental mitochondrial (mt) genomes were used to characterize the novel mt genome present in a somatic hybrid plant. A cosmid clone containing mtDNA restriction fragments characteristic of both parental plant lines was identified in a library constructed from mtDNA of progeny of a somatic hybrid plant. Restriction mapping revealed the location of several restriction fragments derived from each of the parental mt genomes on the same cloned region of somatic hybrid mtDNA. This result is direct evidence that intergenomic recombination at the molecular level occurs in homologous regions of two parental mt genomes combined in the same plant cell by protoplast fusion.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00381175
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  • 6
    Electronic Resource
    Electronic Resource
    Localization of tRNA genes on the Petunia hybrida 3704 mitochondrial genome (1993)
    Springer
    Plant molecular biology 21 (1993), S. 403-407 
    Details
    ISSN: 1573-5028
    Keywords: plant mitochondria ; tRNA genes ; Petunia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract 22 tRNA genes corresponding to 17 tRNA species were localized on the master circle of Petunia hybrida mitochondrial (mt) DNA. Genes for trnN, trnM, trnS-GGA, trnW and trnH are of the ‘chloroplast-like’ type and presumably originate from promiscuous chloroplast (cp) DNA sequences inserted into the petunia mitochondrial genome. A comparison of the mt tRNAs or tRNA genes population present in two monocotyledonous plants (wheat and maize) and two dicotyledonous plants (petunia and potato) show slight differences in the genetic origin of individual tRNAs. The organization of the petunia mt tRNA genes as well as the number of tRNA gene copies, compared to other plant species, is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00019957
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  • 7
    Electronic Resource
    Electronic Resource
    Differential fate of plastid and mitochondrial genomes in Petunia somatic hybrids (1986)
    Springer
    Theoretical and applied genetics 72 (1986), S. 748-755 
    Details
    ISSN: 1432-2242
    Keywords: Petunia ; Chloroplast DNA ; Mitochondrial DNA ; Somatic hybrid ; Protoplast fusion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The chloroplast (cp) and mitochondrial (mt) DNAs of Petunia somatic hybrid plants, which were derived from the fusion of wild-type P. parodii protoplasts with albino P. inflata protoplasts, were analyzed by endonuclease restriction and Southern blot hybridization. Using 32P-labelled probes that distinguished the two parental cpDNAs at a BamH1 site and at a HpaII site, only the P. parodii chloroplast genome was detected in the 10 somatic hybrid plants analyzed. To examine whether cytoplasmic mixing had resulted in rearrangement of the mitochondrial genome in the somatic hybrids, restriction patterns of purified somatic hybrid and parental mtDNAs were analyzed. Approximately 87% of those restriction fragments which distinguish the two parental genomes are P. inflata-specific. Restriction patterns of the somatic hybrid mtDNAs differ both from the parental patterns and from each other, suggesting that an interaction occurred between the parental mitochondrial genomes in the somatic fusion products which resulted in generation of the novel mtDNA patterns. Southern blot hybridization substantiates this conclusion. In addition, somatic hybrid lines derived from the same fusion product were observed to differ in mtDNA restriction pattern, reflecting a differential sorting-out of mitochondrial genomes at the time the plants were regenerated.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00266540
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  • 8
    Electronic Resource
    Electronic Resource
    Different transcript abundance of two divergent ATP synthase subunit 9 genes in the mitochondrial genome of Petunia hybrida (1987)
    Springer
    Molecular genetics and genomics 209 (1987), S. 21-27 
    Details
    ISSN: 1617-4623
    Keywords: ATPase subunit 9 ; Plant mitochondrial genes ; Petunia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The mitochondrial genome of Petunia hybrida line 3704 has been found to contain two transcribed genes for the proteolipid subunit of the ATP synthase complex (atp 9). The 5′ and 3′ flanking sequences of the second atp 9 gene differ from those of another atp 9 gene previously sequenced by Young et al. (1986), while the coding region exhibits only one silent base change. The transcript termini of the two divergent atp 9 genes map to different locations, most of which are surrounded by sequences homologous to putative mitochondrial transcription signals (Hiesel and Brennicke 1985; Schuster et al. 1986; Young et al. 1986). Because transcripts from the atp 9 genes differ in abundance, divergence in the flanking regions of these two genes must affect regulatory elements which control either transcription rate or transcript stability.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00329831
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  • 9
    Electronic Resource
    Electronic Resource
    A NADH dehydrogenase subunit gene is co-transcribed with the abnormal Petunia mitochondrial gene associated with cytoplasmic male sterility (1989)
    Springer
    Molecular genetics and genomics 215 (1989), S. 332-336 
    Details
    ISSN: 1617-4623
    Keywords: NADH dehydrogenase subunit 3 ; Cytoplasmic male sterility ; Petunia ; Co-transcription
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary DNA sequence analysis 3′ to the Petunia S-pcf coding region has resulted in the identification of an open reading frame similar to mammalian mitochondrial genes for subunit 3 of the NADH dehydrogenase complex (nad3). Both the abnormal fused gene S-pcf and S-nad3 fall within the mitochondrial DNA region previously shown to be associated with cytoplasmic male sterility (CMS). The S-nad3 sequence, co-transcribed with S-pcf, is present in only one copy within the Petunia CMS genome. A homologous transcribed sequence from the mitochondrial genome of a fertile Petunia line has been identified. The coding region of the two genes are identical and they share homology for at least 800 bp downstream. The genes diverge 117 bp upstream of the nad3 start codon. Transcripts of the S-pcf/S-nad3 transcripts are similar in tissues of a fertility-restored line and a CMS line.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00339738
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  • 10
    Electronic Resource
    Electronic Resource
    How do alterations in plant mitochondrial genomes disrupt pollen development? (1995)
    Springer
    Journal of bioenergetics and biomembranes 27 (1995), S. 447-457 
    Details
    ISSN: 1573-6881
    Keywords: Mitochondria ; respiration ; pollen development ; Petunia ; cytoplasmic male sterility ; tissue printing ; fluorescence microscopy ; meiosis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Physics
    Notes: Abstract Cytoplasmic male sterility arises when mitochondrial activities are disrupted that are essential for pollen development. Rearrangements in the mitochondrial genome that create novel open reading frames are strongly correlated with CMS phenotypes in a number of systems. The morphological aberrations which indicate CMS-associated degeneration are frequently restricted to the male sporogenous tissue and a limited number of vegetative tissues. In several cases, this tissue specificity may result from interactions between the mitochondrial genome and nuclear genes that regulate mitochondrial gene expression. A molecular mechanism by which CMS might be caused has not been conclusively demonstrated for any system. Several hypotheses for general mechanisms by which mitochondrial dysfunction might disrupt pollen development are discussed, based on similarities between the novel CMS-associated genes from a number of systems.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02110007
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