ISSN:
1573-2592
Keywords:
Phorbol ester
;
mixed lymphocyte reaction
;
leukemia
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract The tumor promoter 12-O-tetradecanoylphorbol-13-acetate (TPA) induces a variety of phenotypic changes on normal and malignant cells. In chronic and acute lymphocytic leukemia we have observed a marked augmentation in mixed lymphocyte reaction stimulatory capacity (MLRs) following pretreatment of leukemia blasts with TPA. We have now characterized the effects of TPA on MLRs utilizing the non-T, non-B lymphoblastic leukemiacell line, HOON. Following pretreatment with TPA (optimal concentration, 1.6×10−10 M), but not with other phorbol esters, there was a marked increase in MLRs, particularly at lower stimulator-cell concentrations. This effect was maximal following a 44-hr preincubation period and the enhancement in MLRs was not accompanied by changes in levels of Ia expression. This cell line provides a model for determining the molecular basis for the TPA-induced augmentation of stimulatory capacity in the mixed lymphocyte reaction.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00914970
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