ISSN:
1617-4623
Keywords:
seg-3 mutant
;
F plasmid
;
Plasmid maintenance
;
Heat shock response
;
Phosphate transport system
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary The seg-3 mutant Escherichia coli does not support the maintenance of mini-F plasmid at 42° C. We cloned the chromosomal DNA segment of the wild-type strain W3110 that complements the Seg− phenotype of this mutant. Cleavage mapping of this segment showed that it was derived from the 76-min region of the E. coli chromosome map. Complementation tests using plasmids carrying subcloned DNA segments suggested that the seg-3 mutant carried two mutations that additively affected the maintenance of mini-F plasmid; one was in the ugpA gene and the other was presumably in the rpoH gene. We generated a disrupted ugpA null mutant and found that the mini-F plasmid was unstable in this ugpA null mutant even at 30° C. This suggests that the ugpA gene product is required for the stable maintenance of mini-F plasmid.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00264441
Permalink
