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  • 1
    Electronic Resource
    Electronic Resource
    Efficient translation of long-lived messengers in extracts from dry pea primary axes (1980)
    Springer
    Planta 150 (1980), S. 286-290 
    Details
    ISSN: 1432-2048
    Keywords: Cell-free translation ; Lectin ; Long-lived mRNA ; Pisum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Extracts prepared from dry pea (Pisum sativum, L; cv oberon) primary axes translate efficiently their endogenous messengers in an in vitro protein synthesizing system. The native long-lived messengers are biologically fully active and direct the synthesis of a whole range of polypeptides with MW ranging up to 130,000. About 0.5% of the total in vitro synthesized polypeptides are recovered in the immunoprecipitate obtained with pea lectin antiserum. Since about one-fourth of the radioactivity in the immunoprecipitate comigrates with authentic pea lectin it is concluded that about 0.1% of the long-lived messengers code for the lectin.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00384657
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Trehalose metabolism in dormant and activated spores of Phycomyces blakesleeanus Burgeff (1978)
    Springer
    Planta 139 (1978), S. 171-176 
    Details
    ISSN: 1432-2048
    Keywords: Dormancy ; Phycomyces ; Spores ; Trehalose
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Evidence is obtained for the existence of two different localizations of trehalase (α,α-trehalose glucohydrolase, EC 3.2.1.28) in Phycomyces spores: one inside the cell, and one in the periplasmic region. The latter enzyme is sensitive to 0.1 mol l-1 HCl treatment and its activity can be regulated by external pH changes. The periplasmic form of the enzyme is involved in the metabolism of added labelled trehalose. This sugar is hydrolyzed externally to glucose which is found mainly in the incubation medium and which is partly absorbed by the spores. During incubation trehalose leaks out from both dormant and activated spores and is subsequently hydrolyzed to glucose. The intracellular trehalase is probably involved in the breakdown of endogenous trehalose in spores. After heat activation the hydrolysis of endogenous trehalose is stimulated even without an important increase in activity of intracellular trehalase. Additional treatments which break dormancy of spores without a significant activation of trehalase are the following: heating of HCl-treated spores and treatment of spores with reducing substances (e.g. Na2S2O4 and NaHSO3).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00387144
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Cell-free translation of exogenous mRNA in extracts from dry pea primary axes (1980)
    Springer
    Planta 147 (1980), S. 302-306 
    Details
    ISSN: 1432-2048
    Keywords: mRNA ; Protein synthesis ; Pisum ; Seeds (translation) ; Translation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Extracts from the primary axes of dry pea (Pisum sativum L.) seeds are able to perform an initiation-dependent translation of exogenous mRNA. SDS polyacrylamide gel electrophoresis of the products synthesized under direction of alfalfa mosaic virus RNA (AMV-RNA) and tobacco mosaic virus RNA (TMV-RNA) shows that the fidelity of translation in this pea system is at least as high as in a wheat embryo cell-free protein synthesizing system. The endogenous messengers are also efficiently translated in extracts from the primary axes of pea seeds. The direct translation of these messengers in a homologous cell-free system may be of interest for a study of the products coded for by the long-lived messengers present in this plant.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00379837
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Synthesis and proteolytic activation of chitin synthetase in Phycomyces blakesleeanus Burgeff (1978)
    Springer
    Archives of microbiology 116 (1978), S. 181-184 
    Details
    ISSN: 1432-072X
    Keywords: Phycomyces ; Chitin synthetase ; Germination ; Proteolytic activation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The chitin synthetase of Phycomyces blakesleeanus mycelium is a particulate enzyme sedimenting mostly at 1000xg. The activity in crude extracts or cellular fractions can be increased more than tenfold by mild trypsin treatment. Plotting the reaction velocity versus UDP-N-acetylglucosamine concentration yields a sigmoidal curve. N-acetylglucosamine, which greatly stimulates the enzyme, changes the kinetics to an almost normal hyperbolic relationship. The enzyme is nearly absent in dormant spores and is synthesized “de novo” in germinating spores (from 4 h germination on). Trypsin treatment of extracts from germinating spores to assay the synthesis of the proenzyme did not reveal an earlier synthesis of the zymogen, which therefore might have some activity of its own.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00406034
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    Density changes of Phycomyces spores after reversible and irreversible activation (1980)
    Springer
    Archives of microbiology 124 (1980), S. 289-291 
    Details
    ISSN: 1432-072X
    Keywords: Phycomyces ; Spores ; Germination ; Activation ; Density gradient centrifugation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Spores harvested by conventional methods could be cleaned up considerably using discontinuous density gradients. This method yielded a population of almost entirely viable spores. Germination of the spores resulted in density changes probably due to spore swelling. These changes could be observed as early as 15 min after activation. The usefulness of this method in the study of spore activation was shown by its application to spores germinating after a heat treatment at various temperatures, or to spore reacquiring dormancy by incubation in water after activation at different temperatures. Dormant and activated spores can quickly and easily be separated from mixed populations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00427740
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    Paper (German National Licenses)
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  • 6
    Electronic Resource
    Electronic Resource
    The effect of gamma radiation on breaking of dormancy in Phycomyces spores (1977)
    Springer
    Archives of microbiology 113 (1977), S. 95-97 
    Details
    ISSN: 1432-072X
    Keywords: Phycomyces ; Spore activation ; Dormancy ; Gamma rays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Dormant sporangiospores of Phycomyces blakesleeanus were activated by Cs-137 gamma rays. After a dose of about 300 krad, between 80 and 90% of the spores germinated normally. However, further development of the mycelium was inhibited. Even with dry spores a partial activation was obtained. Activation by gamma rays was not accompanied by an increase in trehalase activity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00428587
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    Paper (German National Licenses)
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