ISSN:
1432-072X
Keywords:
2,4-Dichlorophenoxyacetic acid
;
Biodegradation
;
Pseudomonas cepacia
;
Chloro-aromatics oxidation
;
Physico-chemical effects
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract A Pseudomonas cepacia, designated strain BRI6001, was isolated from peat by enrichment culture using 2,4-dichlorophenoxyacetic acid (2,4-D) as the sole carbon source. BRI6001 grew at up to 13 mM 2,4-D, and degraded 1 mM 2,4-D at an average starting population density as low as 1.5 cells/ml. Degradation was optimal at acidic pH, but could also be inhibited at low pH, associated with chloride release from the substrate, and the limited buffering capacity of the growth medium. The only metabolite detected during growth on 2,4-D was 2,4-dichlorophenol (2,4-DCP), and degradation of the aromatic nucleus was by intradiol cleavage. Growth lag times prior to the on-set of degradation, and the total time required for degradation, were linearly related to the starting population density and the initial 2,4-D concentration. BRI6001, grown on 2,4-D, oxidized a variety of structurally similar chlorinated aromatic compounds accompanied by stoichiometric chloride release.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00276525
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