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  • 1
    Electronic Resource
    Electronic Resource
    Distribution of phytochrome and chlorophyll-a/b-binding-protein mRNAs in etiolated Avena seedlings (1992)
    Springer
    Planta 187 (1992), S. 532-536 
    Details
    ISSN: 1432-2048
    Keywords: Avena (phytochrome) ; Chlorophyll-a/bbinding protein ; Light regulation ; mRNA localization ; Phytochrome
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In 4-d-old dark-grown oat (Avena sativa L.) seedlings, the majority of the type-I-phytochrome (phyA) mRNA was found within 10 mm of the tip of the coleoptile sheath and in the mesocotyl node; almost none was detected in the enclosed primary leaf. In contrast, chlorophyll-a/b-binding-protein (cab) mRNAs were found almost exclusively in the enclosed primary leaf and were barely detectable in total-RNA samples from the coleoptile sheath or mesocotyl node of red-light-treated etiolated seedlings. Separated, dark-grown primary leaves responded to a red-light treatment by increasing cab-mRNA abundance in the absence of the coleoptile sheath or mesocotyl node tissues.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00199973
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Half-lives of oat mRNAs in vivo and in a polysome-based in-vitro system (1993)
    Springer
    Planta 189 (1993), S. 249-256 
    Details
    ISSN: 1432-2048
    Keywords: Actin ; Avena ; Chlorophyll-a/b-binding protein ; mRNA half-life ; Phytochrome ; β-Tubulin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have used a cell-free polysome-based in-vitro mRNA-degradation system to investigate the halflives of plant cell mRNAs. In order to establish the fidelity of the in-vitro system, we used cordycepin to determine the in-vivo half-lives of β-tubulin and actin mRNAs in the primary leaves of 4-d-old etiolated oat (Avena sativa L.) seedlings. The in-vitro rank order of half-lives for phytochrome A (45 min), β-tubulin (105 min), and actin (220 min) mRNAs mimicked the in-vivo rank order. A pulse of red light given to excised etiolated primary leaves caused an in-vivo reduction in the half-life of β-tubulin mRNA. The selectivity of the polysome-based system was further demonstrated by the decrease in the half-life of β-tubulin mRNA (from 105 min to 60 min) induced by a pulse of red light given to the etiolated oat seedlings prior to isolation of polysomes. Red light did not affect the apparent half-lives of phytochrome A or actin mRNAs.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00195084
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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