ISSN:
1432-1424
Keywords:
Pig follicular cells
;
Tissue culture
;
Whole-cell recording
;
Ca current
;
K current
;
Luteinization
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
Abstract We studied membrane currents in granulosa cells (GC), immediately after collection or after variable culture time in the everted-follicle wall or in the monolayer. GC in both systems express an inward calcium current (I Ca) with T-type kinetics and voltage dependence. GC in the everted-follicle culture express an outward potassium current (I K) kinetics, which remains unchanged during three days in culture. I K has delayed-rectifier kinetics, but is insensitive to TEA, 4-AP and apamine. GC in monolayer culture develop a new, inactivating delayed-rectifier potassium current (I nK), which progressively dominates as cells advance from day one to day three in culture. A similar I nK was recorded in large luteal cells. A possible link between luteinization and the appearance of I nK is hypothesized.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00233477
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