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  • cis-parinaric acid  (2)
  • Pigments  (1)
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  • 1
    Electronic Resource
    Electronic Resource
    Fatty acid double bond orientation alters interaction with L-cell fibroblasts (1996)
    Springer
    Molecular and cellular biochemistry 155 (1996), S. 113-119 
    Details
    ISSN: 1573-4919
    Keywords: L-cells ; oleic acid ; cis-parinaric acid ; trans-parinaric acid ; fatty acid ; transport ; fluorescence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract Relatively little is known of fatty acid specificity in cellular fatty acid uptake. In this study L-cells, a fibroblastic cell line with very low levels of endogenous cytosolic fatty acid binding protein, were used to examine the role of cis and trans unsaturation on fatty acid uptake. The fluorescent fatty acids, trans-parinaric acid and cis-parinaric acid, were used as analogs of straight-chain saturated, and kinked-chain unsaturated fatty acids, respectively, in order to evaluate the fatty acid specificity of the uptake system. Parinaric acid is poorly metabolizable; greater than 97% was unesterified while 3H-oleic acid was almost totally metabolized after 30 min uptake. Cis- and trans-parinaric acid uptake was saturable and dependent on the concentration of fatty acid. However, the initial rate and maximal amount of trans-parinaric acid taken up by the L-cells was greater than for cis-parinaric acid under the same conditions. The affinity of L-cell uptake for trans-parinaric acid (Km = 0.12 uM) was 35-fold higher than that for cis-parinaric acid (Km = 4.17 uM) . Based on competition studies with oleic and stearic acids, it was concluded that the cis- and trans-parinaric acid were taken up by the same L-cell fatty acid uptake system. The results suggest that the L-cell fatty acid uptake system has selectivity for straight chain rather than kinked chain unsaturated fatty acids.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00229308
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Fatty acid uptake in diabetic rat adipocytes (1997)
    Springer
    Molecular and cellular biochemistry 167 (1997), S. 51-60 
    Details
    ISSN: 1573-4919
    Keywords: fatty acid ; cis-parinaric acid ; adipocyte ; transport ; diabetes ; insulin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract The effect of diabetic status and insulin on adipocyte plasma membrane properties and fatty acid uptake was examined. Studies with inhibitors and isolated adipocyte ghost plasma membranes indicated 9Z, 11E, 13E, 15Z-octatetraenoic acid (cis-parinaric acid) uptake was protein mediated. Cis-parinaric acid uptake was inhibited by trypsin treatment or incubation with phloretin, and competed with stearic acid. The initial rate, but not maximal uptake, of cis-parinaric acid uptake was enhanced two-fold in adipocytes from diabetic rats. Concomitantly, the structure and lipid composition of adipocyte ghost membranes was dramatically altered. However, the increased initial rate of cis-parinaric acid uptake in the diabetic adipocytes was not explained by membrane alterations or by a two-fold decrease in cytosolic adipocyte fatty acid binding protein (ALBP), unless ALBP stimulated fatty acid efflux. Thus, diabetic status dramatically altered adipocyte fatty acid uptake, plasma membrane structu re, lipid composition, and cytosolic fatty acid binding protein. (Mol Cell Biochem 167: 51-60, 1997)
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006812302485
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Pigment characterisation of suspended matter in association with particulate heavy metal loadings in the German Bight and Elbe Estuary (1995)
    Springer
    Aquatic ecology 29 (1995), S. 303-314 
    Details
    ISSN: 1573-5125
    Keywords: Pigments ; chlorophylls ; carotenoids ; heavy metals ; Cd ; Cu ; Zn ; Pb ; Fe ; Mn ; Elbe
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The pigment fingerprints, determined by HPLC, of suspended matter from different areas of the German Bight and from the Elbe Estuary are presented. These include areas with suspended matter concentrations varying between 10 mg l−1 in the deeper waters in the middle of the Bight and 150 mg l−1 in the Elbe mouth. Pigment data allows the identification of three groups of suspended matter (marine, turbidity zone and Elbe) for the numerous different locations. The changes in pigment concentrations in the suspended matter over tidal cycles are considered. The presence of pigments and pigment breakdown productsi.e. lutein and chlorophyllides is used to assess the possible condition of the phytoplankton present in the suspended matter. These pigment characteristics are compared with the loading of the heavy metals cadmium, copper, manganese, iron, zinc and lead in the particulate matter. It has been demonstrated that the pigments of the planktonic material can be used very effectively to identify different types of suspended matter and that the heavy metal loadings of the suspended matter were significantly correlated with chlorophyll-a.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02084229
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    Paper (German National Licenses)
    Fulltext
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