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  • 1
    Electronic Resource
    Electronic Resource
    Proximity of an ARS consensus sequence to a replication origin of pea (Pisum sativum) (1988)
    Springer
    Plant molecular biology 10 (1988), S. 413-422 
    Details
    ISSN: 1573-5028
    Keywords: replication origin ; ARS ; rDNA ; Pisum sativum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The replication origin (ori-r9) of the 9.0 kb rDNA repeats of pea (Pisum sativum, cv. Alaska) was cloned and found to reside in a 1.5 kb fragment of the non-transcribed spacer region located between the 25 S and 18 S genes. Labeled rDNA rich in replication forks, from cells positioned at the G1/S phase boundary, was used to map ori-r9 by hybridization procedures. Ori-r9 is in a 210-base fragment that is 1.6 kb from the 5′ end of the 18 S gene and about 1.5 kb from the 3′ end of the 25 S gene. The same procedures, using labeled synthetic ARS consensus sequence as a probe, showed than an ARS consensus sequence is located 3′ to ori-r9 in a 710-base fragment. An ARS consensus sequence is, therefore, adjacent to ori-r9 but not coincidental with it.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00014947
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Location of the replication origin in the 9-kb repeat size class of rDNA in pea (Pisum sativum) (1987)
    Springer
    Plant molecular biology 9 (1987), S. 87-95 
    Details
    ISSN: 1573-5028
    Keywords: Pisum sativum ; replication origin ; rDNA ; non-transcribed spacer
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The replication origin of the 9-kb rDNA repeat size class of pea (Pisum sativum cv. Alaska) was identified by benzoylated naphthoylated DEAE-cellulose column chromatography and Southern blotting procedures. The origin is located at or near a 0.19-kb EcoR I fragment in the non-transcribed spacer region between the 25S and 18S rRNA genes. Identification of the origin was based on three criteria: (i) an enrichment of the 0.19-kb fragment in replicating rDNA from asynchronously dividing root meristematic cells, (ii) the scarcity of the 0.19-kb fragment in rDNA from non-dividing carbohydrate starved cells, and (iii) a 60-min periodic enrichment of the 0.19-kb fragment in replicating rDNA that temporally coincides with the sequential initiation of replication of replicon families in synchronized pea root cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00015641
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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