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  • 1
    Electronic Resource
    Electronic Resource
    Skin occlusion: Effect on Pityrosporum orbiculare, skin $$P_{{\text{CO}}_{\text{2}} } $$ , pH, transepidermal water loss, and water content (1983)
    Springer
    Archives of dermatological research 275 (1983), S. 383-387 
    Details
    ISSN: 1432-069X
    Keywords: Skin occlusion ; Pityrosporum orbiculare ; $$P_{{\text{CO}}_{\text{2}} } $$ ; Transepidermal water loss ; Water content
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The effect of 8 days skin occlusion on Pityrosporum orbiculare, bacteria, skin $$P_{{\text{CO}}_{\text{2}} } $$ , pH, transepidermal water loss (TEWL), and water content (WC) was studied. P. orbiculare counts increased from a baseline of 2.1×102/cm2 to 2.3×103/cm2 after 3 days occlusion; bacterial counts increased from 2.9×103/cm2 to 1.8×105/cm2 after 8 days occlusion. pH increased during occlusion from 5.6 to a maximum at day 3 of 6.7; TEWL increased to a maximum of 11.74 g m−2 h− after 3 days occlusion compared with 4.39g m−2 h−1 before. P. orbiculare counts, pH, and TEWL were lower at 8 days than at 3 days occulusion. WC and $$P_{{\text{CO}}_{\text{2}} } $$ remained high after 8 days; relative WC was then 60.6% compared with 52.5% before occlusion; $$P_{{\text{CO}}_{\text{2}} } $$ was 63.1 mm Hg compared with 53.1 mm Hg before occlusion. The increased levels of these factors may partially explain the higher risk of infection in occluded compared with non-occluded skin.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00417338
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  • 2
    Electronic Resource
    Electronic Resource
    Adherence of Pityrosporum orbiculare to human stratum corneum cells (1983)
    Springer
    Archives of dermatological research 275 (1983), S. 246-250 
    Details
    ISSN: 1432-069X
    Keywords: Pityrosporum orbiculare ; Adherence ; Human stratum corneum cells
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary This investigation demonstrates the capacity of Pityrosporum orbiculare to adhere to human stratum corneum cells in vitro; this may be an improtant initial step in its colonization of stratum corneum. Adherence was significantly higher when incubation for 120 min (mean 9.4 adherent yeasts) was compared to 60 min (mean 3.5 adherent yeasts, P〈0.001), but not to 90 min (mean 8.6 adherent yeats, P〉0.05). Adherence was higher at an incubation temperature of 37°C (mean 10.0 adherent yeasts) as compared with 25°C (mean 4.0, P〈0.001), but not at 32°C (mean 8.0, P〉0.1). There was no significant difference in adherence related to the anatomic area (forearm, back, and chest) from which the stratum corneum cells have been collected. No difference was seen in adherence between different Pityrosporum strains, but it increased significantly with inoculum size of P. orbiculare, being optimal at 107 cells ml-1. The adherence of P. orbiculare and Staphylococcus epidermidis to stratum corneum cells was not influenced by incubation of the organisms together or alone. This model opens possibilities to study the influence of: stratum corneum cells from diseased skin compared with controls; antibodies; and types of surface receptors and antimycotics on P. orbiculare adherence to stratum corneum cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00416670
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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