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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Archives of toxicology 39 (1978), S. 267-287 
    ISSN: 1432-0738
    Keywords: Rat ; Plasma ; Alkaline phosphatase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract This report describes the application of a test system which applies different substrates, inhibitors and acrylamide electrophoresis to the study of the alkaline phosphatase activity of rats. The system, which was specifically devised to characterise the alkaline phosphatase activity of small quantities of plasma from rats in toxicological studies, was also used to examine the plasma from rats under several physiological conditions, e.g. time of day, age, diet, etc. These physiological factors can influence the measurement of plasma alkaline phosphatase activity and should be taken into account when designing toxicological studies. Different experimental regimes were used to produce increases in liver, bone and intestinal alkaline phosphatase isoenzymes in the plasma of rats and these were reflected in the characterisations by the system. Although much remains unknown, a few concepts of rat plasma alkaline phosphatase activity emerged under our conditions. There is a ‘tidal’ intestinal component which arises from feeding and is approximately 60% of the total activity of the fed rat. In the fasted rat this ‘tidal’ component has largely disappeared and the ‘core’ activity which remains is composed of approximately equal proportions of bone isoenzyme and another as yet ill-defined component. The liver isoenzyme which appears in the plasma of the cholestatic rat appears to be either a very minor component or absent from the plasma of our normal CFE derived rat.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-0738
    Keywords: Cholinesterase ; Plasma ; Brain ; Carbamate ; Organophosphorus Compounds ; Cholinesterase ; Plasma ; Gehirn ; Carbamate ; Organophosphate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Dieser Report beschreibt die Modifikationen, die notwendig sind, um die pH-Stat und Ellman-Methode für die Bestimmung der Cholinesteraseaktivität im Plasma und Gehirn der verschiedenen Laboratoriumstierarten anzupassen. Beide Methoden wurden durch Untersuchungen bewertet, bei denen den Tieren cholinesterasehemmende Stoffe, Carbamate und organische Phosphorverbindungen, gegeben worden waren.
    Notes: Abstract This report describes the modifications necessary to adapt the pH Stat and Ellman methods for the estimation of cholinesterase activity in the plasma and brain of various species of laboratory animals. Both methods were evaluated in studies in which cholinesterase inhibitors, carbamates and organophosphorus compounds, were given to the animals.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Archives of toxicology 39 (1978), S. 249-266 
    ISSN: 1432-0738
    Keywords: Rat ; Alkaline phosphatase ; Plasma ; Organs ; Toxicity testing
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract A manual system of various estimations of rat plasma alkaline phosphatase activity has been devised for small volumes of plasma which uses different inhibitors, compares the utilisation of two substrates and includes acrylamide gel electrophoresis. The different inhibitors etc. allow a degree of discrimination between alkaline phosphatase extracts of rat organs. The properties of isoenzymes, e.g. intestinal alkaline phosphatase, differ depending upon the environment in which they are studied. In conjunction, if necessary, with the methods described for the estimation of liver and intestinal alkaline phosphatase activity, it is hoped to use the system to discriminate between the isoenzymes present in the plasma alkaline phosphatase of rats in toxicological studies.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Euphytica 29 (1980), S. 369-377 
    ISSN: 1573-5060
    Keywords: Hordeum vulgare ; barley ; Hordeum bulbosum ; bulbous barley grass ; interspecific cross ; incompatibility
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Partial incompatibility has already been reported between Hordeum vulgare and H. bulbosum during the course of doubled haploid production, and in this paper attempts to overcome the breeding barrier are described. The methods which seem to offer most success are those of environmental adjustments and the adoption of new genotypes of H. bulbosum. Further cultivars of H. vulgare exhibiting this phenomenon are also noted.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1573-5060
    Keywords: Hordeum vulgare ; barley ; Hordeum bulbosum ; bulbous barley grass ; embryo culture ; haploids ; hybrids ; plant regeneration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Plant regeneration rates from embryos derived from Hordeum vulgare x H. bulbosum were recorded over a 3 1/2 year period. % total plant regeneration (haploids + VB hybrids) varied to some extent but did not seem to be influenced by season or male parent. % VB production (and thus chromosome elimination) was however markedly affected by season and the genotype of the pollinator.
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1573-5060
    Keywords: Hordeum vulgare ; barley ; Hordeum bulbosum ; bulbous barley grass ; haploid embryos ; variation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Improvements in the success of doubled haploid production have been achieved partly by screening new accessions of Hordeum bulbosum. In order to assess levels of variation within stocks of this species, rates of embryo differentiation from the cross between H. vulgare × H. bulbosum were recorded for selections derived from two different stocks of H. bulbosum. There was little difference within stocks for this character despite variation in the morphology and banding patterns of two enzyme systems of one of the stocks. It is proposed that to obtain further increases in success rates a few selections from many accessions of H. bulbosum should be screened rather than many selections from a few stocks.
    Type of Medium: Electronic Resource
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