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  • 1
    Electronic Resource
    Electronic Resource
    Complementation of the Saccharomyces cerevisiae srb1-1 mutation: an autoselection system for stable plasmid maintenance (1992)
    Springer
    Current genetics 21 (1992), S. 339-344 
    Details
    ISSN: 1432-0983
    Keywords: Yeast ; Saccharomyces cerevisiae ; Lysis mutants ; Plasmid stability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The autonomously replicating plasmid YEpSS1, containing the S. cerevisiae SOD1 and SRB1 genes, was highly unstable in a wild-type strain. When transformed into a fragile srb1-1 mutant host, the same plasmid displayed different characteristics depending on the growth medium used. Both batch and continuous culture experiments demonstrated that the plasmid was very unstable when the transformed strain SLU15 was grown in the presence of an osmotic stabiliser (10% w/v sorbitol). However, in the absence of the osmoticum, nearly 100% of the cells retained the plasmid and produced the Sod1 protein after 80 generations of growth.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00351692
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    The yeast 2 μ plasmid: strategies for the survival of a selfish DNA (1986)
    Springer
    Molecular genetics and genomics 205 (1986), S. 417-421 
    Details
    ISSN: 1617-4623
    Keywords: Plasmid stability ; 2 μ DNA-Saccharomyces ; Chemostat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The designation of the yeast 2 μ circle as a “selfish” DNA molecule has been confirmed by demonstrating that the plasmid is lost with exponential kinetics from haploid yeast populations grown in continuous culture. We show that plasmid-free yeast cells have a growth rate advantage of some 1.5%–3% over their plasmid-containing counterparts. This finding makes the ubiquity of this selfish DNA in yeast strains puzzling. Two other factors probably account for its survival. First, the rate of plasmid loss was reduced by allowing haploid populations to enter stationary phase periodically. Second, it was not possible to isolate a plasmid-free segregant from a diploid yeast strain. Competition experiments demonstrated that stability in a diploid is conferred at the level of segregation and that plasmid-free diploid cells are at a selective advantage compared with their plasmid-containing counterparts. Yeast cells in nature are usually homothallic and must frequently pass through both diploid and stationary phases. The 2 μ plasmid appears to have evolved a survival strategy which exploits these two features of its host's life cycle.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00338076
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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