ISSN:
1573-5044
Keywords:
Campanulaceae
;
Platycodon grandiflorum
;
somatic embryo
;
suspension culture
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Mature zygotic embryos of balloon flower (Platycodon grandiflorum) formed embryogenic calluses at a frequency of 43% when cultured on Murashige and Skoog medium supplemented with 4.52 μM 2,4-dichlorophenoxyacetic acid (2,4-D). Cell suspension cultures were established from embryogenic calluses using MS liquid medium with 4.52 μM 2,4-D. Following transfer to solid MS basal medium, cell suspension cultures gave rise to somatic embryos, which then developed into plantlets. Plantlets were transplanted to potting soil and grown to maturity.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1006338024745
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