ISSN:
0935-6304
Keywords:
Thin-layer chromatography, HPTLC
;
Gradient saturation
;
Polar lipids
;
Soybean lecithin
;
Phospholipids
;
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
A thermostated HPTLC system was assembled to test the influence of gradient chamber saturation upon resolution of polar lipids chromatographed on boric acid-impregnated silica gel plates. Inexpensive mixtures of nonacidic (14 component peaks) and acidic (8 component peaks) lipids were prepared from soya lecithin by a simplified procedure involving DEAE-Sephadex liquid column chromatography to monitor performance of the system using a readily available reference material. The system requires ca. 5 min chamber presaturation after which the polar lipids are chromatographed in less than 30 min. Retention times (tR) as well as peak area % values of all component peaks and measured by scanning photodensitometry to demonstrate the influence of different modes of chamber saturation upon the resolution of polar lopids.
Additional Material:
3 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/jhrc.1240080705
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