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  • 1
    Electronic Resource
    Electronic Resource
    The promoter of a gene that is expressed only in pollen interacts with ubiquitous transcription factors (1996)
    Springer
    Sexual plant reproduction 9 (1996), S. 197-202 
    Details
    ISSN: 1432-2145
    Keywords: Tobacco ; Pollen ; Transcriptional control ; GT-1 · GT-2
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract TheNTP303 gene of tobacco is expressed only in bicellular pollen. The minimal pollen-specific promoter of the gene is located between −103 and −51 upstream of the transcriptional start site. Using gel mobility shift assays, we demonstrated that this minimal functional promoter interacts with a leaf nuclear GT-1 binding activity. This finding suggests that ubiquitous transcription factors are involved in the pollen-specific expression of the gene in vivo.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02173098
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Discrimination of related transcribed and non-transcribed repetitive DNA sequences from the Y chromosomes of Drosophila hydei and Drosophila eohydei (1994)
    Springer
    Molecular genetics and genomics 243 (1994), S. 54-62 
    Details
    ISSN: 1617-4623
    Keywords: Drosophila ; Y chromosome ; Fertility genes Lampbrush loops ; Repetitive DNA sequences
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The short arm of the Y chromosome of Drosophila hydei carries a single male fertility gene, gene Q, which forms the lampbrush loop pair Nooses. Conflicting observations have been reported concerning the identity of the repetitive DNA sequences that are transcribed in this loop pair. It has been claimed by other investigators that the loop transcripts contain repeats of two distinct, but related families of Y-specific repetitive DNA sequences, ayl and YsI. We reinvestigated this issue, using as probes single ayl and YsI repeats which, under stringent conditions, hybridize only to members of their own family. Under non-stringent conditions, both repeats hybridize in situ to Nooses transcripts. However, if hybridization conditions are stringent, only the ayl probe hybridizes to loop transcripts. Hybridizations to Northern blots of testis RNA confirm these results. Further, YsI repeats are not found the closely related species D. eohydei. We conclude that the YsI repeats are not relevant for the function of fertility gene Q.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00283876
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Transcription of repetitive DNA sequences in the lampbrush loop pair Nooses formed by sterile alleles of fertility gene Q on the Y chromosome of Drosophila hydei (1994)
    Springer
    Molecular genetics and genomics 244 (1994), S. 653-660 
    Details
    ISSN: 1617-4623
    Keywords: Drosophila ; Fertility genes ; Y chromosome Lampbrush loops ; Repetitive DNA sequences
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The Y chromosomal lampbrush loop-forming male fertility genes of Drosophila consist mainly of repetitive DNA sequences that do not code for proteins. We investigated whether differences in the transcription of these sequences can be detected in male-sterile alleles of male fertility gene Q, which forms the loop pair Nooses. The loop consists, for approximately two-thirds, of repeats of the Y-specific ay1 family of repetitive DNA sequences. Of the remaining one-third, at least one-half is represented by defective retrotransposons of the gypsy family. Both sequence types are interspersed throughout the loop. Using both ay1 and gypsy sequences as probes for transcript in situ hybridization, we show that, at the level of the light microscope, transcription of neither sequence is detectably affected in the loops formed by a male-sterile allele of gene Q. We conclude that the transcription of ay1 and gypsy is required, but not sufficient for the function of gene Q.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00282756
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    Paper (German National Licenses)
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