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  • 1
    Electronic Resource
    Electronic Resource
    Skeletal muscle oedema and muscle fibre necrosis during septic shock. Observations with a porcine septic shock model (1994)
    Springer
    Virchows Archiv 424 (1994), S. 653-659 
    Details
    ISSN: 1432-2307
    Keywords: Septic shock ; Skeletal muscle ; Porcine shock model
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract In domestic pigs, intermitted application ofEscherichia coli-endotoxin was used to create an animal model for a prolonged hypo- and hyperdynamic septic shock-like state and to investigate mechanisms of multiple organ failure. Here, we describe the changes in skeletal muscle after 18 h (2 animals) and 48 h (6 animals) of septic shock. Two pigs for each observation period that received physiologic saline solutions instead of endotoxin served as controls. The earliest lesions were endothelial cell damage with endomysial oedema and swelling of mitochondria in muscle fibres. With increasing degree of endothelial cell damage, pericytes showed degenerative changes with cytoplasmic fragmentation and karyolysis. After 48 h of shock, endomysial oedema was increased with fibrinogen present. Muscle fibre diameters were increased and swollen mitochondria and segmental necrosis of muscle fibres were frequently observed. However, phagocytic reaction or regenerative changes were not detected. In this respect, skeletal muscle lesions in septic shock differ from ischemic damage, which is characterized by early phagocytosis. Tumour necrosis factor alpha (TNFα) was increased greatly and significantly in the serum of the pigs that received endotoxin. The lesions described may be the result of both direct damage to muscle fibres by the endotoxin and/or the increased levels of TNFα and indirect damage because of the increased diffusion distance, due to the endomysial oedema. The loss of blood proteins into the endomysium may also play a role in generating hypoproteinemia in patients with septic shock.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01069747
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  • 2
    Electronic Resource
    Electronic Resource
    Skeletal muscle oedema and muscle fibre necrosis during septic shock. Observations with a porcine septic shock model (1994)
    Springer
    Virchows Archiv 424 (1994), S. 653-659 
    Details
    ISSN: 1432-2307
    Keywords: Septic shock ; Skeletal muscle ; Porcine shock model
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract In domestic pigs, intermitted application of Escherichia coli-endotoxin was used to create an animal model for a prolonged hypo- and hyperdynamic septic shock-like state and to investigate mechanisms of multiple organ failure. Here, we describe the changes in skeletal muscle after 18 h (2 animals) and 48 h (6 animals) of septic shock. Two pigs for each observation period that received physiologic saline solutions instead of endotoxin served as controls. The earliest lesions were endothelial cell damage with endomysial oedema and swelling of mitochondria in muscle fibres. With increasing degree of endothelial cell damage, pericytes showed degenerative changes with cytoplasmic fragmentation and karyolysis. After 48 h of shock, endomysial oedema was increased with fibrinogen present. Muscle fibre diameters were increased and swollen mitochondria and segmental necrosis of muscle fibres were frequently observed. However, phagocytic reaction or regenerative changes were not detected. In this respect, skeletal muscle lesions in septic shock differ from ischemic damage, which is characterized by early phagocytosis. Tumour necrosis factor alpha (TNFα) was increased greatly and significantly in the serum of the pigs that received endotoxin. The lesions described may be the result of both direct damage to muscle fibres by the endotoxin and/or the increased levels of TNFα and indirect damage because of the increased diffusion distance, due to the endomysial oedema. The loss of blood proteins into the endomysium may also play a role in generating hypoproteinemia in patients with septic shock.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00195781
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Morphology of cardiac muscle in septic shock. Observations with a porcine septic shock model (1995)
    Springer
    Virchows Archiv 426 (1995), S. 487-491 
    Details
    ISSN: 1432-2307
    Keywords: Cardiac muscle ; Microvasculature ; Septic shock ; Porcine shock model
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The morphology of cardiac muscle was investigated in a porcine model of septic shock, created by intermitted application of Escherichia coli-endotoxin. The earliest lesions, found after 18 h of septic shock, were endothelial cell swelling, marked leucostasis and slight ischaemic alterations of the muscle fibres. At the end point of the experiments, after 48 h, some fibrin thrombi were found associated with more pronounced ischaemic alterations of cardiac muscle cells and some necrotic fibres. Comparing these findings with the severe endothelial and muscle fibre lesions found in skeletal muscle, the endothelial cells of the heart microvasculature, are clearly more resistant to the attack of the endotoxins and mediators liberated in septic shock.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00193172
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  • 4
    Electronic Resource
    Electronic Resource
    Modifizierung von polymeroberflächen zur erhöhung der zelladhäusionVortrag anläßlich der Tagung der Fachgruppe “Makromolekulare Chemie” der Gesellschaft Deutscher Chemiker über “Polymere und biologische Funktionen” in Bad Nauheim am 18. und 19. April 1988 (1989)
    Weinheim : Wiley-Blackwell
    Angewandte Makromolekulare Chemie 166 (1989), S. 179-189 
    Details
    ISSN: 0003-3146
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Notes: After implantation a stable bond between the implant and the surrounding tissue is required. Therefore a high cell adhesion of the polymer surface of the implant must be achieved. Depending on the treatment time of a polydimethylsiloxane foil with oxygen plasma, the cell adhesion can be improved. FT-IR spectroscopy and ESCA analysis were used to characterize the surface modification. The cell spreading and cell adhesion increase with increasing hydrophilic character of the polymer surface after plasma treatment. A pronounced correlation was found between the efficiency of DNA and protein content, characterizing cell growth, and the spreading of the cells.Polydimethylsiloxane, Glow-Discharge, Surface Modification, Cell Adhesion, Cell Proliferation.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/apmc.1989.051660113
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  • 5
    Electronic Resource
    Electronic Resource
    Surface analysis of PP/EVA blends modified with amino acids for biomedical applications (1995)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Polymer Science 58 (1995), S. 699-710 
    Details
    ISSN: 0021-8995
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: The copolymers poly(propylene-co-ethylene) (PP/E) and poly(ethylene-co-vinyl acetate) (EVA) and blends of these were modified to develop an artificial matrix which promotes the growth of endothelial cells. Covalent immobilization of amino acids or sequences of adhesion glycoproteins should trigger the formation of an endothelial cell monolayer onto the polymeric surface. Reactive functional groups were generated by saponifying the ester groups of the EVA component. Esterification with oxalylic or malonic dichlorides in the gas phase yielded the required monoesters and gave the best results for further immobilization of amino acids, while reaction with α,ω-dicarboxylic acid dichlorides in solution led to diester formation. Subsequently, various protected amino acids were immobilized via the carbodiimide method. Surface analytical methods like infrared spectroscopy using at tenuated total reflection (IR-ATR), X-ray photoelectron spectroscopy (XPS), and secondary ion mass spectrometry (SIMS) were used to prove the modification steps. The analytical results confirmed covalent side-chain generation in the upper surface region. © 1995 John Wiley & Sons, Inc.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/app.1995.070580403
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    Paper (German National Licenses)
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