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  • 1
    Electronic Resource
    Electronic Resource
    Phylogenetic relationships amongTetrahymena species determined using the polymerase chain reaction (1990)
    Springer
    Journal of molecular evolution 30 (1990), S. 290-297 
    Details
    ISSN: 1432-1432
    Keywords: Tetrahymena ; Phylogenies ; Polymerase chain reaction ; Histones ; Macronuclear DNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The species of theTetrahymena pyriformis complex present a conundrum with regard to their highly conservative morphology and widely divergent molecular characteristics. We have investigated the phylogenetic relationships among these species using the nucleotide sequences from the histone H3II/H4II region of the genome. This region includes portions of the two histone coding sequences, as well as the intergenic region. The DNA sequences of these regions were amplified by the polymerase chain reaction (PCR) and the sequence of each was determined. Nucleotide substitutions and insertions/deletions within this set of sequences were compared to determine the phylogenetic relationships among the species of the complex. These data yield phylogenetic trees with identical topologies when different tree-building routines are used, indicating that the data are very robust.Glaucoma chattoni was used as an outgroup to root the trees for this analysis. The genome organization ofG. chattoni and the divergence of its histone H3II/H4II region sequence relative to those of the complex clearly indicate that this species has diverged considerably from the complex. These results show that PCR amplification analysis is feasible over considerable evolutionary distances. However, DNA-DNA hybridization may be more useful than sequence analysis in resolving the relationships among the closely related species in the complex.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02099999
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  • 2
    Electronic Resource
    Electronic Resource
    The nucleotide sequence of the small subunit ribosomal RNA gene from Symbiodinium pilosum, a symbiotic dinoflagellate (1992)
    Springer
    Current genetics 21 (1992), S. 409-416 
    Details
    ISSN: 1432-0983
    Keywords: Molecular phylogeny ; Dinoflagellates ; Polymerase chain reaction ; Small subunit ribosomal RNA gene ; Symbiodinium pilosum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The complete sequence of the small subunit ribosomal RNA (SSU rRNA) gene was determined for the symbiotic dinoflagellate Symbiodinium pilosum. This sequence was compared with sequences from two other dinoflagellates (Prorocentrum micans and Crypthecodinium cohnii), five Apicomplexa, five Ciliata, five other eukaryotes and one archaebacterium. The corresponding structurally conserved regions of the molecule were used to determine which portions of the sequences could be unambiguously aligned. Phylogenetic relationships were inferred from an analysis of distance matrices, where pair-wise distances were determined using a maximum likelihood model for transition and transversion ratios, and from maximum parsimony analysis, with bootstrap resampling. By either analytical approach, the dinoflagellates appear distantly related to prokaryotes, and are most closely related to two of the Apicomplexa, Sarcocystis muris and Theileria annulata. Among the dinoflagellates, C. cohnii was found to be more closely affiliated with the Apicomplexa than either P. micans or S. pilosum.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00351703
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  • 3
    Electronic Resource
    Electronic Resource
    Variable copy number of macronuclear DNA molecules in Tetrahymena (1992)
    Chichester [u.a.] : Wiley-Blackwell
    Developmental Genetics 13 (1992), S. 111-117 
    Details
    ISSN: 0192-253X
    Keywords: Tetrahymena ; copy number ; histone H4 ; macronuclear DNA molecules ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: In Tetrahymena, the DNA of the macronucleus exists as very large (100 to 4,000-kb) linear molecules that are randomly partitioned to the daughter cells during cell division. This genetic system leads directly to an assortment of alleles such that all loci become homozygous during vegetative growth. Apparently, there is a copy number control mechanism operative that adjusts the number of each macronuclear DNA molecule so that macronuclear DNA molecules (with their loci) are not lost and aneuploid death is a rare event. In comparing Southern analyses of the DNA from various species of Tetrahymena using histone H4 genes as a probe, we find different band intensities in many species. These differences in band intensities primarily reflect differences in the copy number of macronuclear DNA molecules. The variation in copy number of macronuclear DNA molecules in some species is greater than an order of magnitude. These observations are consistent with a developmental control mechanism that operates by increasing the macronuclear copy number of specific DNA molecules (and the genes located on these molecules) to provide the relatively high gene copy number required for highly expressed proteins. © 1992 Wiley-Liss, Inc.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/dvg.1020130204
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