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  • 1
    Electronic Resource
    Electronic Resource
    Lack of mutations of the adenomatous polyposis coli gene in oesophageal and gastric carcinomas (1994)
    Springer
    Virchows Archiv 424 (1994), S. 607-611 
    Details
    ISSN: 1432-2307
    Keywords: Adenomatous polyposis coli gene ; Gastrointestinal tract carcinoma ; Mutation ; Polymerase chain reaction ; Tumour suppressor gene
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The adenomatous polyposis coli (APC) gene is the target of the loss of chromosome 5q heterozygosity observed frequently in gastrointestinal tract carcinomas and is inactivated in these carcinomas. We screened 94 gastrointestinal tract carcinomas forAPC mutations, by polymerase chain reaction single-strand conformation polymorphism (SSCP) analysis. Mutations were detected in 8 of 21 (38%) colorectal carcinomas in the mutation cluster region of theAPC gene whereas no mutation was detected in any of 49 oesophageal and 24 gastric carcinomas, even though SSCP analysis was extended to include the 5′ half of theAPC gene exon 15. Direct DNA sequencing revealed that six of eight (75%) mutations in colorectal carcinomas resulted in truncated gene products. These findings confirm the significance ofAPC gene mutations in colorectal, but not oesophageal or gastric carcinomas. Some other tumour suppressor genes near theAPC gene may be the target of the frequent allelic loss of chromosome 5q in oesophageal and gastric carcinomas.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01069740
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Lack of mutations of the adenomatous polyposis coli gene in oesophageal and gastric carcinomas (1994)
    Springer
    Virchows Archiv 424 (1994), S. 607-611 
    Details
    ISSN: 1432-2307
    Keywords: Adenomatous polyposis coli gene ; Gastrointestinal tract carcinoma ; Mutation ; Polymerase chain reaction ; Tumour suppressor gene
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The adenomatous polyposis coli (APC) gene is the target of the loss of chromosome 5q heterozygosity observed frequently in gastrointestinal tract carcinomas and is inactivated in these carcinomas. We screened 94 gastrointestinal tract carcinomas for APC mutations, by polymerase chain reaction single-strand conformation polymorphism (SSCP) analysis. Mutations were detected in 8 of 21 (38%) colorectal carcinomas in the mutation cluster region of the APC gene whereas no mutation was detected in any of 49 oesophageal and 24 gastric carcinomas, even though SSCP analysis was extended to include the 5′ half of the APC gene exon 15. Direct DNA sequencing revealed that six of eight (75%) mutations in colorectal carcinomas resulted in truncated gene products. These findings confirm the significance of APC gene mutations in colorectal, but not oesophageal or gastric carcinomas. Some other tumour suppressor genes near the APC gene may be the target of the frequent allelic loss of chromosome 5q in oesophageal and gastric carcinomas.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00195774
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Analysis of genetic alterations in renal cell carcinoma using the polymerase chain reaction (1994)
    Springer
    Virchows Archiv 424 (1994), S. 453-457 
    Details
    ISSN: 1432-2307
    Keywords: Tumour suppressor genes ; ras oncogene Renal cell carcinoma ; Polymerase chain reaction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Very frequent loss of heterozygosity (LOH) on chromosome 3p has been found in human renal cell carcinoma (RCC). In the present study, we examined LOH at the retinoblastoma (RB), mutated in colorectal cancer (MCC) and adenomatous polyposis coli (APC) tumour suppressor genes loci, and mutations of the H-, K-, and N-ras oncogenes. We performed these studies using the polymerase chain reaction (PCR) method followed by restriction fragment length polymorphism (RFLP) and single-strand conformation polymorphism (SSCP) analyses. LOH was detected in 2 of 11 (18.2%), and 2 of 14 (14.3%) informative cases at the MCC and APC loci, respectively, and in none of 15 informative cases at the RB locus in 25 RCCs. LOH at the MCC was accompanied by LOH at the APC locus in two RCCs. No mutation was detected in H-, K-, and N-ras genes in 39 RCCs. Thus, alterations of the known tumour suppressor genes and the ras oncogenes were infrequent events in RCC. The results suggest that the genetic pathway in the genesis of RCC differs considerably from that of other common human carcinomas.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00191428
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
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